Literature DB >> 6252099

Separation of human eosinophils in density gradients of polyvinylpyrrolidone-coated silica gel (Percoll).

I Gärtner.   

Abstract

A method for isolation of eosinophils from human peripheral blood using isosmolar solutions of polyvinylpyrrolidone-coated silica gel (Percoll) is described. The purity ranged from 86 to 99% eosinophils in the final preparation and the recovery was 38-56%. The separation technique did not affect the viability or the metabolic capacities of the cells.

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Year:  1980        PMID： 6252099      PMCID： PMC1458478     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  9 in total

1.  A method for obtaining concentrates of eosinophils from blood.

Authors:  R F Alexander; A I Spriggs
Journal:  J Clin Pathol       Date:  1962-03       Impact factor: 3.411

2.  Oxidative metabolism of the human eosinophil.

Authors:  L R DeChatelet; P S Shirley; L C McPhail; C C Huntley; H B Muss; D A Bass
Journal:  Blood       Date:  1977-09       Impact factor: 22.113

3.  Myeloperoxidase-mediated iodination in granulocytes.

Authors:  I Olsson; T Olofsson; H Odeberg
Journal:  Scand J Haematol       Date:  1972

4.  Production of monospecific rabbit antihuman eosinophil serums and demonstration of a blocking phenomenon.

Authors:  A A Mahmoud; R W Kellermeyer; K S Warren
Journal:  N Engl J Med       Date:  1974-02-21       Impact factor: 91.245

5.  Selective stimulation and purification of eosinophils and neutrophils from guinea pig peritoneal fluids.

Authors:  G J Gleich; D Loegering
Journal:  J Lab Clin Med       Date:  1973-09

6.  Eosinophil cell separation from human peripheral blood.

Authors:  R P Day
Journal:  Immunology       Date:  1970-06       Impact factor: 7.397

7.  Characterization of functionally distinct lymphoid and myeloid cells from human blood and bone marrow. I. Separation by a buoyant density gradient technique.

Authors:  J R Wells; G Opelz; M J Cline
Journal:  J Immunol Methods       Date:  1977       Impact factor: 2.303

8.  Granulocyte function in chronic granulocytic leukemia. II. Bactericidal capacity, phagocytic rate, oxygen consumption, and granule protein composition in isolated granulocytes.

Authors:  T Olofsson; H Odeberg; I Olsson
Journal:  Blood       Date:  1976-10       Impact factor: 22.113

9.  Separation of HeLa cells by colloidal silica density gradient centrifugation. I. Separation and partial synchrony of mitotic cells.

Authors:  D A Wolff; H Pertoft
Journal:  J Cell Biol       Date:  1972-12       Impact factor: 10.539
  9 in total
  31 in total

1.  A comparison of the inhibitory activity of PDE4 inhibitors on leukocyte PDE4 activity in vitro and eosinophil trafficking in vivo.

Authors:  N Cooper; M M Teixeira; J Warneck; J M Miotla; R E Wills; D M Macari; R W Gristwood; P G Hellewell
Journal:  Br J Pharmacol       Date:  1999-04       Impact factor: 8.739

2.  Isolation of human eosinophils.

Authors:  K G Lim; P F Weller
Journal:  Curr Protoc Immunol       Date:  2001-05

3.  Priming effect of platelet activating factor on leukotriene C4 from stimulated eosinophils of asthmatic patients.

Authors:  K Shindo; K Koide; Y Hirai; M Sumitomo; M Fukumura
Journal:  Thorax       Date:  1996-02       Impact factor: 9.139

Review 4.  Eosinophils. Assays and interpretation.

Authors:  D E Maddox
Journal:  Clin Rev Allergy       Date:  1988

5.  An extragranular compartment of blood eosinophils contains eosinophil protein X/eosinophil-derived neurotoxin (EPX/EDN).

Authors:  Malgorzata Karawajczyk; Christer G B Peterson; Per Venge; Rodolfo C Garcia
Journal:  Inflammation       Date:  2013-04       Impact factor: 4.092

6.  Purification of eosinophils from normal human blood, preparation of eosinoplasts and characterization of their functional response to various stimuli.

Authors:  M Yazdanbakhsh; C M Eckmann; M De Boer; D Roos
Journal:  Immunology       Date:  1987-01       Impact factor: 7.397

7.  Generation and metabolism of 5-lipoxygenase pathway leukotrienes by human eosinophils: predominant production of leukotriene C4.

Authors:  P F Weller; C W Lee; D W Foster; E J Corey; K F Austen; R A Lewis
Journal:  Proc Natl Acad Sci U S A       Date:  1983-12       Impact factor: 11.205

8.  Phospholipid analysis of human eosinophils: high levels of alkylacylglycerophosphocholine (PAF precursor).

Authors:  A Ojima-Uchiyama; Y Masuzawa; T Sugiura; K Waku; H Saito; Y Yui; H Tomioka
Journal:  Lipids       Date:  1988-08       Impact factor: 1.880

9.  Human eosinophils and parasitic diseases. II. Characterization of two cell fractions isolated at different densities.

Authors:  C De Simone; G Donneli; D Meli; F Rosati; F Sorice
Journal:  Clin Exp Immunol       Date:  1982-04       Impact factor: 4.330

10.  Inhibition of eosinophil cyclic nucleotide PDE activity and opsonised zymosan-stimulated respiratory burst by 'type IV'-selective PDE inhibitors.

Authors:  G Dent; M A Giembycz; K F Rabe; P J Barnes
Journal:  Br J Pharmacol       Date:  1991-06       Impact factor: 8.739
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