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Literature DB >> 18432843

Isolation of human eosinophils.

Abstract

Highly purified eosinophils can be isolated from peripheral blood by negative selection using anti-CD16 immunomagnetic beads. The basic protocol describes a sequential fractionation of peripheral blood in which eosinophils are separated first from mononuclear cells and then from CD16(+) neutrophils. This technique is easy to use, fast, and highly reproducible. A support protocol describes a staining method that can be used to evaluate the purity of eosinphils.

Entities: Gene Species

Mesh：

Substances：
Receptors, IgG

Year: 2001 PMID： 18432843 PMCID： PMC3420812 DOI： 10.1002/0471142735.im0731s20

Source DB: PubMed Journal: Curr Protoc Immunol ISSN： 1934-3671

20 in total

1. High gradient magnetic cell separation with MACS.

Authors: S Miltenyi; W Müller; W Weichel; A Radbruch
Journal: Cytometry Date: 1990

2. Isolation of human eosinophils.

Authors: K G Lim; P F Weller
Journal: Curr Protoc Immunol Date: 2001-05

3. Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3.

Authors: M Kitaura; T Nakajima; T Imai; S Harada; C Combadiere; H L Tiffany; P M Murphy; O Yoshie
Journal: J Biol Chem Date: 1996-03-29 Impact factor: 5.157

4. Effect of isolation protocol on eosinophil function: Percoll gradients versus immunomagnetic beads.

Authors: J B Sedgwick; Y Shikama; M Nagata; K Brener; W W Busse
Journal: J Immunol Methods Date: 1996-10-30 Impact factor: 2.303

5. Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily.

Authors: H Floyd; J Ni; A L Cornish; Z Zeng; D Liu; K C Carter; J Steel; P R Crocker
Journal: J Biol Chem Date: 2000-01-14 Impact factor: 5.157

6. Eosinophil cell separation from human peripheral blood.

Authors: R P Day
Journal: Immunology Date: 1970-06 Impact factor: 7.397

7. Separation of human eosinophils in density gradients of polyvinylpyrrolidone-coated silica gel (Percoll).

Authors: I Gärtner
Journal: Immunology Date: 1980-05 Impact factor: 7.397

8. Ammonium chloride exposure inhibits cytokine-mediated eosinophil survival.

Authors: M Ide; D Weiler; H Kita; G J Gleich
Journal: J Immunol Methods Date: 1994-02-10 Impact factor: 2.303

9. Discontinuous Percoll gradient centrifugation combined with immunomagnetic separation obviates the need for erythrocyte lysis and yields isolated eosinophils with minimal granule abnormalities.

Authors: Monika Malm-Erjefält; Tim R Stevens; Carl G A Persson; Jonas S Erjefält
Journal: J Immunol Methods Date: 2004-05 Impact factor: 2.303