Literature DB >> 6249806

Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA.

J P Ford, M Coca-Prados, M T Hsu.   

Abstract

An enzymatic method is described for the analysis of the content of 5-methylcytosine in the DNA of eukaryotic cells. The conventional acid hydrolysis procedure was found to result in variable deamination of 5-methylcytosine into thymine, making the exact quantitation difficult. We applied the enzymatic method for the analysis of a simple eukaryotic viral genome. Viral and cell DNA in Simian Virus 40-infected monkey kidney cells was labeled with [methyl-3H]methionine in vivo and their 5-methylcytosine content was analyzed. The content of 5-methylcytosine per thymine in the total intracellular viral DNA was found to be less than 10(-4) of the host DNA. The implication of this result with regard to eukaryotic gene expression is discussed.

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Year:  1980        PMID： 6249806

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  9 in total

1.  Methylated DNA-binding protein is present in various mammalian cell types.

Authors:  P C Supakar; D Weist; D L Zhang; N Inamdar; X Y Zhang; R Khan; K C Ehrlich; M Ehrlich
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

2.  Hypomethylation of Epstein-Barr virus DNA in the nonproducer B-cell line EBR.

Authors:  D Larocca; W Clough
Journal:  J Virol       Date:  1982-09       Impact factor: 5.103

3.  Amount and distribution of 5-methylcytosine in human DNA from different types of tissues of cells.

Authors:  M Ehrlich; M A Gama-Sosa; L H Huang; R M Midgett; K C Kuo; R A McCune; C Gehrke
Journal:  Nucleic Acids Res       Date:  1982-04-24       Impact factor: 16.971

4.  UV-induced photoproducts of 5-methylcytosine in a DNA sequence context.

Authors:  T Barna; J Malinowski; P Holton; M Ruchirawat; F F Becker; J N Lapeyre
Journal:  Nucleic Acids Res       Date:  1988-04-25       Impact factor: 16.971

5.  Epstein-Barr HR-1 virion DNA is very highly methylated.

Authors:  E S Diala; R M Hoffman
Journal:  J Virol       Date:  1983-01       Impact factor: 5.103

6.  Ethylation of poly(dC-dG).poly(dC-dG) by ethyl methanesulfonate stimulates the activity of mammalian DNA methyltransferase in vitro.

Authors:  I K Farrance; R Ivarie
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

7.  Incorporation of 5-substituted analogs of deoxycytidine into DNA of herpes simplex virus-infected or - transformed cells without deamination to the thymidine analog.

Authors:  L Fox; M J Dobersen; S Greer
Journal:  Antimicrob Agents Chemother       Date:  1983-03       Impact factor: 5.191

8.  Joint meeting of the British and European Associations for Cancer Research and the Royal Society of Medicine (Section of Oncology). November 3-5, 1986, London. Abstracts.

Authors: 
Journal:  Br J Cancer       Date:  1987-03       Impact factor: 7.640

9.  CpG distribution and methylation pattern in porcine parvovirus.

Authors:  Renáta Tóth; István Mészáros; Rajmund Stefancsik; Dániel Bartha; Ádám Bálint; Zoltán Zádori
Journal:  PLoS One       Date:  2013-12-31       Impact factor: 3.240
  9 in total

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