Literature DB >> 3856245

Ethylation of poly(dC-dG).poly(dC-dG) by ethyl methanesulfonate stimulates the activity of mammalian DNA methyltransferase in vitro.

I K Farrance, R Ivarie.   

Abstract

Ethylation of poly(dC-dG).poly(dC-dG) with ethyl methanesulfonate (EtMes), a known carcinogen, at increasing molar ratios of EtMes/C X G base pairs progressively stimulated the methyl-accepting ability of the DNA during in vitro methylation by partially purified rat DNA (cytosine-5)-methyltransferase (EC 2.1.1.37). Maximum stimulation was 2-fold over mock-treated DNA when 2.7% of the guanines were modified at the N-7 position, the major site of ethylation by EtMes in DNA. If a CpG site "hemiethylated" at guanine N-7 mimics a hemimethylated CpG site, we calculate that the enzyme has a relative affinity for hemiethylated CpG 18-fold above unmodified CpG. If ethylation of a dioxyphosphate oxygen of the phosphodiester bond is responsible for stimulation, the relative affinity could be much higher, up to 370-fold.

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Year:  1985        PMID： 3856245      PMCID： PMC397190          DOI： 10.1073/pnas.82.4.1045

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  34 in total

Review 1.  X inactivation, differentiation, and DNA methylation.

Authors:  A D Riggs
Journal:  Cytogenet Cell Genet       Date:  1975

2.  DNA modification mechanisms and gene activity during development.

Authors:  R Holliday; J E Pugh
Journal:  Science       Date:  1975-01-24       Impact factor: 47.728

3.  The specificity of different classes of ethylating agents toward various sites of HeLa cell DNA in vitro and in vivo.

Authors:  L Sun; B Singer
Journal:  Biochemistry       Date:  1975-04-22       Impact factor: 3.162

4.  DNA methylase: purification from ascites cells and the effect of various DNA substrates on its activity.

Authors:  J F Turnbull; R L Adams
Journal:  Nucleic Acids Res       Date:  1976-03       Impact factor: 16.971

5.  Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

Authors:  J D Sharp; N E Capecchi; M R Capecchi
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

6.  Reaction of guanosine with ethylating agents.

Authors:  B Singer
Journal:  Biochemistry       Date:  1972-10-10       Impact factor: 3.162

Review 7.  DNA methylation and gene activity.

Authors:  W Doerfler
Journal:  Annu Rev Biochem       Date:  1983       Impact factor: 23.643

8.  Mouse DNA methylase: methylation of native DNA.

Authors:  R L Adams; E L McKay; L M Craig; R H Burdon
Journal:  Biochim Biophys Acta       Date:  1979-02-27

9.  DNA-methylase from regenerating rat liver: purification and characterisation.

Authors:  D Simon; F Grunert; U von Acken; H P Döring; H Kröger
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971

10.  Effect of a single treatment with the alkylating carcinogens dimethylnitrosamine and methyl methanesulphonate on liver regenerating after partial hepatectomy. IV. Effect on methylase-mediated methylation of DNA.

Authors:  V M Craddock; A R Henderson
Journal:  Chem Biol Interact       Date:  1979-09       Impact factor: 5.192
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  9 in total

1.  Activation of two new alpha(1,3)fucosyltransferase activities in Chinese hamster ovary cells by 5-azacytidine.

Authors:  B Potvin; P Stanley
Journal:  Cell Regul       Date:  1991-12

2.  Methylation patterns of HLA-DR alpha genes in six mononuclear cell lines.

Authors:  Y Wang; B M Peterlin
Journal:  Immunogenetics       Date:  1986       Impact factor: 2.846

3.  Mechanism of human methyl-directed DNA methyltransferase and the fidelity of cytosine methylation.

Authors:  S S Smith; B E Kaplan; L C Sowers; E M Newman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-15       Impact factor: 11.205

4.  Supercoiling-dependent sequence specificity of mammalian DNA methyltransferase.

Authors:  T Bestor
Journal:  Nucleic Acids Res       Date:  1987-05-11       Impact factor: 16.971

Review 5.  Can cancer chemotherapy enhance the malignant behaviour of tumours?

Authors:  T J McMillan; I R Hart
Journal:  Cancer Metastasis Rev       Date:  1987       Impact factor: 9.264

6.  Site-specific methylation in gene coding region underlies transcriptional silencing of the Phytochrome A epiallele in Arabidopsis thaliana.

Authors:  Gulab Rangani; Mariya Khodakovskaya; Mohammad Alimohammadi; Ute Hoecker; Vibha Srivastava
Journal:  Plant Mol Biol       Date:  2012-04-01       Impact factor: 4.076

7.  Chemical carcinogen-induced decreases in genomic 5-methyldeoxycytidine content of normal human bronchial epithelial cells.

Authors:  V L Wilson; R A Smith; J Longoria; M A Liotta; C M Harper; C C Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

8.  Activation of a nonexpressed hypoxanthine phosphoribosyltransferase allele in mutant H23 HeLa cells by agents that inhibit DNA methylation.

Authors:  R Ivarie; J A Morris
Journal:  Mol Cell Biol       Date:  1986-01       Impact factor: 4.272

9.  Azacytidine-induced reactivation of a DNA repair gene in Chinese hamster ovary cells.

Authors:  P A Jeggo; R Holliday
Journal:  Mol Cell Biol       Date:  1986-08       Impact factor: 4.272
  9 in total

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