| Literature DB >> 2615760 |
K Spanier1, J Schell, P H Schreier.
Abstract
The physiological function in planta of T-DNA gene 6b was studied under various experimental conditions. For this purpose the coding region of gene 6b was cloned behind the 1'-promoter of the TR-DNA to enhance expression of the gene product in transformed plant cells. Expression of the recombinant gene in leaf discs of Nicotiana tabacum altered the capacity for shoot formation of the discs, induced by exogenous (i.e. BAP in the growth medium or agrobacterial trans-zeatin produced under control of gene tzs) or endogenous cytokinins (i.e. isopentenyladenosine produced under control of T-DNA gene 4). The data obtained indicate a reduction of cytokinin activity within the plant cells by the product of T-DNA gene 6b.Entities:
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Year: 1989 PMID： 2615760 DOI： 10.1007/bf00261179
Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925