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Literature DB >> 6235236

Concentrations of high-mobility-group proteins in the nucleus and cytoplasm of several rat tissues.

Abstract

Nuclear and cytoplasmic fractions were isolated from various tissues of the rat by a nonaqueous technique. The high-mobility-group (HMG) proteins were extracted from these fractions with acid and separated by one- and two-dimensional PAGE. The concentrations of high-mobility-group proteins HMG1, HMG2, and HMG17 in the nucleus and cytoplasm were then estimated from the staining intensities of the electrophoretic bands. The cytoplasmic concentrations of these proteins were very low--usually less than 1/30 of those present in the corresponding nuclear fractions. For the tissues studied (liver, kidney, heart, and lung), the concentrations of HMG proteins in the nucleus did not differ significantly from one tissue to another. Averaged over the four tissues investigated, there were 0.28 molecule of HMG1, 0.18 molecule of HMG2, and 0.46 molecule of HMG17 per nucleosome. These values are considerably higher than those that have been reported previously.

Entities: Gene Species

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Year: 1984 PMID： 6235236 PMCID： PMC2113252 DOI： 10.1083/jcb.99.2.648

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

35 in total

1. An improved large scale fractionation of high mobility group non-histone chromatin proteins.

Authors: G H Goodwin; R H Nicolas; E W Johns
Journal: Biochim Biophys Acta Date: 1975-10-20

2. Antibodies against chromosomal HMG proteins stain the cytoplasm of mammalian cells.

Authors: M Bustin; N K Neihart
Journal: Cell Date: 1979-01 Impact factor: 41.582

3. Immunochemical studies of high mobility group non-histone chromatin proteins HMG 1 and HMG 2.

Authors: B J Smith; D Robertson; M S Birbeck; G H Goodwin; E W Johns
Journal: Exp Cell Res Date: 1978-09 Impact factor: 3.905

4. A comparison of the high mobility group non-histone chromatin protein HMG 2 in chicken thymus and erythrocytes.

Authors: C G Mathew; G H Goodwin; K Gooderham; J M Walker; E W Johns
Journal: Biochem Biophys Res Commun Date: 1979-04-27 Impact factor: 3.575

5. Microinjection of the nonhistone chromosomal protein HMG1 into bovine fibroblasts and HeLa cells.

Authors: M Rechsteiner; L Kuehl
Journal: Cell Date: 1979-04 Impact factor: 41.582

6. Limited action of micrococcal nuclease on trout testis nuclei generates two mononucleosome subsets enriched in transcribed DNA sequences.

Authors: B Levy-Wilson; G H Dixon
Journal: Proc Natl Acad Sci U S A Date: 1979-04 Impact factor: 11.205

7. The partial amino acid sequence of a non-histone chromosomal protein.

Authors: J M Walker; J R Hastings; E W Johns
Journal: Biochem Biophys Res Commun Date: 1976-11-08 Impact factor: 3.575

8. Levels of chromosomal protein high mobility group 2 parallel the proliferative activity of testis, skeletal muscle, and other organs.

Authors: S M Seyedin; W S Kistler
Journal: J Biol Chem Date: 1979-11-25 Impact factor: 5.157

9. Distribution of high mobility group proteins among domains of trout testis chromatin differing in their susceptibility to micrococcal nuclease.

Authors: L Kuehl; T Lyness; G H Dixon; B Levy-Wilson
Journal: J Biol Chem Date: 1980-02-10 Impact factor: 5.157

10. Subnucleosome particles containing high mobility group proteins HMG-E and HMG-G originate from transcriptionally active chromatin.

Authors: V V Bakayev; V V Schmatchenko; G P Georgiev
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

22 in total

1. Solution structure of the HMG protein NHP6A and its interaction with DNA reveals the structural determinants for non-sequence-specific binding.

Authors: F H Allain; Y M Yen; J E Masse; P Schultze; T Dieckmann; R C Johnson; J Feigon
Journal: EMBO J Date: 1999-05-04 Impact factor: 11.598

2. Arabidopsis chromatin-associated HMGA and HMGB use different nuclear targeting signals and display highly dynamic localization within the nucleus.

Authors: Dorte Launholt; Thomas Merkle; Andreas Houben; Alexander Schulz; Klaus D Grasser
Journal: Plant Cell Date: 2006-11-17 Impact factor: 11.277

3. A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1.

Authors: W Kruger; I Herskowitz
Journal: Mol Cell Biol Date: 1991-08 Impact factor: 4.272

4. High mobility group proteins cHMG1a, cHMG1b, and cHMGI are distinctly distributed in chromosomes and differentially expressed during ecdysone dependent cell differentiation.

Authors: S Ghidelli; P Claus; G Thies; J R Wiśniewski
Journal: Chromosoma Date: 1997-04 Impact factor: 4.316

Review 5. Chromatin: constructing the big picture.

Authors: Bas van Steensel
Journal: EMBO J Date: 2011-04-28 Impact factor: 11.598

6. Evolution of high mobility group nucleosome-binding proteins and its implications for vertebrate chromatin specialization.

Authors: Rodrigo González-Romero; José M Eirín-López; Juan Ausió
Journal: Mol Biol Evol Date: 2014-10-03 Impact factor: 16.240

7. Phosphorylation and subcellular redistribution of high mobility group proteins 14 and 17, analyzed by mass spectrometry.

Authors: D F Louie; K K Gloor; S C Galasinski; K A Resing; N G Ahn
Journal: Protein Sci Date: 2000-01 Impact factor: 6.725