Literature DB >> 6232323

High resolution isoelectric focusing of immunoprecipitated proteins under denaturing conditions. A simple analytical method applied to the study of complement component polymorphisms.

S Rodriguez de Córdoba, P Rubinstein, A Ferreira.   

Abstract

A simple analytical method for the study of structural protein polymorphisms is described. It consists of the immunoprecipitation of non-radiolabeled proteins using monospecific polyclonal antibodies followed by isoelectric focusing (IEF) under completely denaturing conditions in vertical polyacrylamide slab gels. The method uses small amounts of sample (usually unfractionated plasma or serum), requires no sophisticated equipment and allows the screening of large numbers of samples with comparatively small effort. This method has been applied in the identification of 2 human complement-component polymorphisms, C4-binding protein (C4-bp) and factor H (beta 1H).

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Year:  1984        PMID： 6232323     DOI： 10.1016/0022-1759(84)90314-4

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  11 in total

1.  Mechanisms of thermoinactivation of endoglucanase I from Trichoderma reesei QM 9414.

Authors:  J M Dominguez; C Acebal; J Jimenez; I de la Mata; R Macarron; M P Castillon
Journal:  Biochem J       Date:  1992-10-15       Impact factor: 3.857

2.  Polymorphism and deficiency of human factor H-related proteins p39 and p37.

Authors:  E Feifel; W M Prodinger; M Mölgg; W Schwaeble; D Schönitzer; V Koistinen; R Misasi; M P Dierich
Journal:  Immunogenetics       Date:  1992       Impact factor: 2.846

3.  Epstein-Barr virus/complement fragment C3d receptor (CR2) reacts with p53, a cellular antioncogene-encoded membrane phosphoprotein: detection by polyclonal anti-idiotypic anti-CR2 antibodies.

Authors:  M Barel; A Fiandino; F Lyamani; R Frade
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

4.  Coagulation factor XIII B subunit is encoded by a gene linked to the regulator of complement activation (RCA) gene cluster in man.

Authors:  S Rodriguez de Cordoba; J Rey-Campos; D D Dykes; P J McAlpine; P Wong; P Rubinstein
Journal:  Immunogenetics       Date:  1988       Impact factor: 2.846

5.  New alleles of C4-binding protein and factor H and further linkage data in the regulator of complement activation (RCA) gene cluster in man.

Authors:  S Rodriguez de Cordoba; P Rubinstein
Journal:  Immunogenetics       Date:  1987       Impact factor: 2.846

6.  Evidence for linkage between the loci coding for the binding protein for the fourth component of human complement (C4BP) and for the C3b/C4b receptor.

Authors:  S Rodriguez de Cordoba; T R Dykman; F Ginsberg-Fellner; G Ercilla; M Aqua; J P Atkinson; P Rubinstein
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

7.  Does the mouse C4-binding protein gene (C4BP) map in the H-2 region?

Authors:  S Rodríguez de Córdoba; A Ferreira; P Rubinstein
Journal:  Immunogenetics       Date:  1985       Impact factor: 2.846

8.  Production of a monoclonal antibody directed against an interferon-induced 56,000-dalton protein and its use in the study of this protein.

Authors:  B Y Rubin; S L Anderson; R M Lunn; G R Hellermann; N K Richardson; L J Smith
Journal:  J Virol       Date:  1988-06       Impact factor: 5.103

9.  Decay-accelerating factor. Genetic polymorphism and linkage to the RCA (regulator of complement activation) gene cluster in humans.

Authors:  J Rey-Campos; P Rubinstein; S Rodriguez de Cordoba
Journal:  J Exp Med       Date:  1987-07-01       Impact factor: 14.307

10.  Human genes for three complement components that regulate the activation of C3 are tightly linked.

Authors:  S Rodriguez de Cordoba; D M Lublin; P Rubinstein; J P Atkinson
Journal:  J Exp Med       Date:  1985-05-01       Impact factor: 14.307
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