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Literature DB >> 6205969

5-Azacytidine-induced undercondensations in human chromosomes.

Abstract

The cytosine analogue 5-azacytidine induces very distinct undercondensations in human chromosomes if applied to lymphocyte cultures. The number of induced undercondensations and their chromosomal localization can be varied by the 5-azacytidine dose and the treatment time. "Pulverized" chromosomes or undercondensations in the G-band-positive chromosome regions are produced with high doses and long treatment times. If applied in low doses during the last hours of culture, 5-azacytidine induces specific undercondensations in the heterochromatin of chromosomes 1, 9, 15, 16, and Y. Optimum conditions required for inducing the various types of undercondensation in the chromosomes were determined. Various examples of the use of 5-azacytidine in the analysis of chromosome rearrangements involving heterochromatic regions are presented.

Entities: Chemical Species

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Year: 1984 PMID： 6205969 DOI： 10.1007/bf00291352

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

14 in total

1. A simple technique for demonstrating centromeric heterochromatin.

Authors: A T Sumner
Journal: Exp Cell Res Date: 1972-11 Impact factor: 3.905

2. The structure of DNA from Escherichia coli cultured in the presence of 5-azacytidine.

Authors: S Zadrazil; V Fucík; P Bartl; Z Sormová; F Sorm
Journal: Biochim Biophys Acta Date: 1965-12-09

3. Cell cycle-specific changes in the ultrastructural organization of prematurely condensed chromosomes.

Authors: S K Hanks; S M Gollin; P N Rao; W Wray; W N Hittelman
Journal: Chromosoma Date: 1983 Impact factor: 4.316

4. Segmentation of human chromosomes induced by 5-ACR (5-azacytidine).

Authors: E Viegas-Péquignot; B Dutrillaux
Journal: Hum Genet Date: 1976-12-15 Impact factor: 4.132

5. The location of four human satellite DNAs on human chromosomes.

Authors: J R Gosden; A R Mitchell; R A Buckland; R P Clayton; H J Evans
Journal: Exp Cell Res Date: 1975-04 Impact factor: 3.905

6. Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A.

Authors: D Schweizer; P Ambros; M Andrle
Journal: Exp Cell Res Date: 1978-02 Impact factor: 3.905

Review 7. Heterochromatin and satellite DNA in man: properties and prospects.

Authors: G L Miklos; B John
Journal: Am J Hum Genet Date: 1979-05 Impact factor: 11.025

8. 5-Methylcytosine in heterochromatic regions of chromosomes: chimpanzee and gorilla compared to the human.

Authors: W Schnedl; V G Dev; R Tantravahi; D A Miller; B F Erlanger; O J Miller
Journal: Chromosoma Date: 1975-09-15 Impact factor: 4.316

9. Comparative analysis of karyotypes in European shrew species. II. Constitutive heterochromatin, replication patterns, and sister chromatid exchanges in Sorex araneus and S. gemellus.

Authors: M Schmid; W Schempp; J Olert
Journal: Cytogenet Cell Genet Date: 1982

10. A direct demonstration of somatically paired heterochromatin of human chromosomes.

Authors: M Schmid; D Grunert; T Haaf; W Engel
Journal: Cytogenet Cell Genet Date: 1983

44 in total

1. Kinetochore formation in experimentally undercondensed chromosomes.

Authors: T Haaf; M Schmid
Journal: Hum Genet Date: 1990-05 Impact factor: 4.132

2. Oncogenes result in genomic alterations that activate a transcriptionally silent, dominantly selectable reporter gene (neo).

Authors: R E Drews; V T Chan; L E Schnipper
Journal: Mol Cell Biol Date: 1992-01 Impact factor: 4.272

5-Azacytidine-induced undercondensations in human chromosomes.

1. A simple technique for demonstrating centromeric heterochromatin.

2. The structure of DNA from Escherichia coli cultured in the presence of 5-azacytidine.

3. Cell cycle-specific changes in the ultrastructural organization of prematurely condensed chromosomes.

4. Segmentation of human chromosomes induced by 5-ACR (5-azacytidine).

5. The location of four human satellite DNAs on human chromosomes.

6. Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A.

Review 7. Heterochromatin and satellite DNA in man: properties and prospects.

8. 5-Methylcytosine in heterochromatic regions of chromosomes: chimpanzee and gorilla compared to the human.

9. Comparative analysis of karyotypes in European shrew species. II. Constitutive heterochromatin, replication patterns, and sister chromatid exchanges in Sorex araneus and S. gemellus.

10. A direct demonstration of somatically paired heterochromatin of human chromosomes.

1. Kinetochore formation in experimentally undercondensed chromosomes.

2. Oncogenes result in genomic alterations that activate a transcriptionally silent, dominantly selectable reporter gene (neo).

Review 3. Epigenetic factors in aging and longevity.

4. Inhibition of condensation in human chromosomes induced by the thymidine analogue 5-iododeoxyuridine.

5. An extra band within the human 9qh+ region that behaves like the surrounding constitutive heterochromatin.

6. 5-azacytidine produces differential undercondensation of alpha, beta and classical human satellite DNAs.

7. Rare variants of chromosome 9 with extra G positive band within the qh region are not alike.

8. Developmental abnormalities and epimutations associated with DNA hypomethylation mutations.

9. Centromeric association and non-random distribution of centromeres in human tumour cells.

10. Cytological evidence for 5-azacytidine-induced demethylation of the heterochromatic regions of human chromosomes.