Literature DB >> 6202720

Method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens.

J Tavares de Castro, J F San Miguel, J Soler, D Catovsky.   

Abstract

A method for the simultaneous labelling of terminal deoxynucleotidyl transferase (TdT) and membrane antigens is described. TdT is visualised in the cell nucleus with the peroxidase-antiperoxidase (PAP) method, and the immunogold method is used in combination with monoclonal antibodies against membrane antigens. The morphology of the labelled cells is well preserved for analysis by light microscopy and the preparations obtained can be kept permanently. This method is useful for the analysis of mixed cell proliferations, particularly in leukaemias.

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Year:  1984        PMID： 6202720      PMCID： PMC498835          DOI： 10.1136/jcp.37.6.628

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  14 in total

1.  The detection of intracellular antigens in human leucocytes by immunoperoxidase staining.

Authors:  D Y Mason; C Farrell; C R Taylor
Journal:  Br J Haematol       Date:  1975-11       Impact factor: 6.998

2.  Immunogold staining method for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies: its application to the enumeration of lymphocyte subpopulations.

Authors:  M De Waele; J De Mey; M Moeremans; M De Brabander; B Van Camp
Journal:  J Histochem Cytochem       Date:  1983-03       Impact factor: 2.479

3.  Cytochemistry of normal lymphocyte subsets defined by monoclonal antibodies and immunocolloidal gold.

Authors:  A Crockard; D Catovsky
Journal:  Scand J Haematol       Date:  1983-05

4.  A differentiation antigen expressed selectively by a proportion of human blood cells: detection with a monoclonal antibody.

Authors:  D A Brooks; J Bradley; H Zola
Journal:  Pathology       Date:  1982-01       Impact factor: 5.306

5.  Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression.

Authors:  E L Reinherz; P C Kung; J M Pesando; J Ritz; G Goldstein; S F Schlossman
Journal:  J Exp Med       Date:  1979-12-01       Impact factor: 14.307

6.  Antibody to terminal deoxynucleotidyl transferase.

Authors:  F J Bollum
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

7.  Monoclonal antibodies against antigens of the human platelet surface: preparation and properties.

Authors:  H Zola; P J McNamara; I G Beckman; D A Brooks; P J Macardle; J Bradley; K Goodall; J Skinner; R Collins; M C Berndt
Journal:  Pathology       Date:  1984-01       Impact factor: 5.306

8.  A monoclonal antibody to human acute lymphoblastic leukaemia antigen.

Authors:  J Ritz; J M Pesando; J Notis-McConarty; H Lazarus; S F Schlossman
Journal:  Nature       Date:  1980-02-07       Impact factor: 49.962

9.  Terminal deoxynucleotidyl transferase as a hematopoietic cell marker.

Authors:  F J Bollum
Journal:  Blood       Date:  1979-12       Impact factor: 22.113

10.  Phorbol ester-induced differentiation of a non-T, non-B leukemic cell line: model for human lymphoid progenitor cell development.

Authors:  T W LeBien; F J Bollum; W G Yasmineh; J H Kersey
Journal:  J Immunol       Date:  1982-03       Impact factor: 5.422
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