Literature DB >> 2453027

Preparation of covalently linked DNA-RNA hybrids and arabinocytidine containing DNA fragments.

E de Vroom1, H C Roelen, C P Saris, T N Budding, G A van der Marel, J H van Boom.   

Abstract

It will be demonstrated that 5'-O-DMT-N-acyl-deoxyribonucleosides, 5'-O-Lev-2'-O-MTHP-N-acyl-ribonucleosides and, also, 2'-O-MTHP-N-acyl-ara-cytidine can be coupled, via the hydroxybenzotriazole phosphotriester approach, to afford two types of DNA-RNA hybrids as well as ara-C containing DNA-fragments. The final removal of acid-labile DMT and MTHP groups could be effected by 1 h treatment with 80% acetic acid of the otherwise unprotected DNA-RNA hybrids. The same acidic hydrolysis did not result in complete removal of the 2'-O-MTHP group from the ara-C unit. Complete deblocking was accomplished after an additional 2 h aqueous HC1 (0.01 M; pH 2.00) treatment.

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Year:  1988        PMID： 2453027      PMCID： PMC336446          DOI： 10.1093/nar/16.7.2987

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  21 in total

1.  The synthesis of oligoribonucleotides. II. Methoxymethylidene derivatives of ribonucleosides and 5'-ribonucleotides.

Authors:  B E Griffin; M Jarman; C B Reese; J E Sulston
Journal:  Tetrahedron       Date:  1967-05       Impact factor: 2.457

2.  Contribution to the mechanism of the acid-catalyzed hydrolysis of purine nucleosides.

Authors:  L Hevesi; E Wolfson-Davidson; J B Nagy; O B Nagy; A Bruylants
Journal:  J Am Chem Soc       Date:  1972-06-28       Impact factor: 15.419

3.  The synthesis of oligoribonucleotides. IV. Preparation of dinucleoside phosphates from 2',5'-protected ribonucleoside derivatives.

Authors:  B E Griffin; M Jarman; C B Reese
Journal:  Tetrahedron       Date:  1968-01       Impact factor: 2.457

Review 4.  Discontinuous DNA replication.

Authors:  T Ogawa; T Okazaki
Journal:  Annu Rev Biochem       Date:  1980       Impact factor: 23.643

5.  RNA-linked nascent DNA fragments in Escherichia coli.

Authors:  A Sugino; S Hirose; R Okazaki
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205

6.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

7.  Chemical synthesis of a messenger ribonucleic acid fragment: AUGUUCUUCUUCUUCUUC.

Authors:  J A den Hartog; G Wille; R A Scheublin; J H van Boom
Journal:  Biochemistry       Date:  1982-03-02       Impact factor: 3.162

8.  Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.

Authors:  K Miyoshi; T Huang; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

9.  Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters.

Authors:  C B Reese; L Zard
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

10.  Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Authors:  M L Duckworth; M J Gait; P Goelet; G F Hong; M Singh; R C Titmas
Journal:  Nucleic Acids Res       Date:  1981-04-10       Impact factor: 16.971
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  1 in total

1.  Chemical synthesis of DNA oligomers containing cytosine arabinoside.

Authors:  G P Beardsley; T Mikita; M M Klaus; A L Nussbaum
Journal:  Nucleic Acids Res       Date:  1988-10-11       Impact factor: 16.971
  1 in total

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