Human beta-globin promoter and coding sequences transcribed by RNA polymerase III.

We have investigated low abundance RNAs transcribed in vitro and in vivo from the human beta-globin gene. These RNAs contain globin mRNA sequences covalently linked to sequences transcribed from the 5' flanking region between -235 and the mRNA cap site (+1). Their synthesis in vitro is sensitive to high (100 micrograms/ml) levels of alpha-amanitin but not to low (2 micrograms/ml) levels, and one region of the DNA template bordering their 5' termini is similar to a small segment of Alu repetitive DNA and to the RNA polymerase III promoter consensus sequence. Therefore, these RNAs are transcribed by RNA polymerase III but extend into the mRNA-coding region that is usually transcribed by polymerase II. The polymerase III transcripts are polyadenylated and are probably spliced. Their presence in bone marrow cells and peripheral blood reticulocytes implies that they play some role in the erythroid cell.