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Literature DB >> 24302159

Specific transcription of a 15-kilodalton zene gene in HeLa cell extracts.

Abstract

A maize genomic clone containing a 15 kilodalton zein gene was used as a template in an in vitro transcription system for HeLa cells. A runoff assay indicated transcription was initiating 5' to the map position of the open reading frame for the protein. Fine-structure mapping of RNAs synthesized in vitro showed two transcription start sites separated by 24 bases. One start site is 27 bases downstream of a consensus TATA sequence; the other is 30 bases downstream of a TATG sequences. The initiation sites for RNA synthesized in vitro map to the same region of the genomic clone as zein RNA isolated from developing maize kernels.

Entities: Species

Year: 1986 PMID： 24302159 DOI： 10.1007/BF00020133

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

25 in total

1. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors: A J Berk; P A Sharp
Journal: Cell Date: 1977-11 Impact factor: 41.582

2. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

3. An improved method for the rapid isolation of brain ribonucleic acid.

Authors: B B Kaplan; S L Bernstein; A E Gioio
Journal: Biochem J Date: 1979-10-01 Impact factor: 3.857

4. Transcription of a gene for human U1 small nuclear RNA.

Authors: J T Murphy; R R Burgess; J E Dahlberg; E Lund
Journal: Cell Date: 1982-05 Impact factor: 41.582

5. A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

Authors: B Wasylyk; P Chambon
Journal: Nucleic Acids Res Date: 1981-04-24 Impact factor: 16.971

6. Complex organization of zein genes in maize.

Authors: G Hagen; I Rubenstein
Journal: Gene Date: 1981-04 Impact factor: 3.688

7. Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNA.

Authors: J Brady; M Radonovich; M Vodkin; V Natarajan; M Thoren; G Das; J Janik; N P Salzman
Journal: Cell Date: 1982-12 Impact factor: 41.582

Specific transcription of a 15-kilodalton zene gene in HeLa cell extracts.

1. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

2. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

3. An improved method for the rapid isolation of brain ribonucleic acid.

4. Transcription of a gene for human U1 small nuclear RNA.

5. A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

6. Complex organization of zein genes in maize.

7. Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNA.

8. Genetic regulation of storaage protein content in maize endosperm.

9. Human beta-globin promoter and coding sequences transcribed by RNA polymerase III.

10. Quantitative analysis of the accumulation of Zein mRNA during maize endosperm development.

1. A homologous expression system for cloned zein genes.