Literature DB >> 6189482

The purification of a novel amylase from Bacillus subtilis and its inhibition by wheat proteins.

A R Orlando, P Ade, D Di Maggio, C Fanelli, L Vittozzi.   

Abstract

A new alpha-amylase (EC 3.2.1.1) from Bacillus subtilis was purified by affinity chromatography. The molecular weight of the purified enzyme, estimated from sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, was 93000, which is very different from the molecular weights of two well-characterized amylases from B. subtilis. Electrofocusing showed an isoelectric point of 5. Amylase shows a broad maximum of activity between pH 6 and 7; maximal inhibition of enzyme by wheat-protein alpha-amylase inhibitors is displayed at pH 7.

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Year:  1983        PMID： 6189482      PMCID： PMC1154127          DOI： 10.1042/bj2090561

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Interaction of Tenebrio molitor L. alpha-amylase with a wheat flour protein inhibitor.

Authors:  V Buonocore; E Poerio
Journal:  FEBS Lett       Date:  1976-08-15       Impact factor: 4.124

2.  DETERMINATION OF AN EQUILIBRIUM CONSTANT FOR THE DIMERIZATION OF THE BACILLUS SUBTILIS ALPHA-AMYLASE MOLECULE.

Authors:  K KAKIUCHI
Journal:  J Phys Chem       Date:  1965-06

3.  A model for the interaction of wheat monomeric and dimeric protein inhibitors with alpha-amylase.

Authors:  V Silano; E Poerio; V Buonocore
Journal:  Mol Cell Biochem       Date:  1977-12-29       Impact factor: 3.396

4.  Purification and properties of an alpha-amylase inhibitor from wheat.

Authors:  M D O'Donnell; K F McGeeney
Journal:  Biochim Biophys Acta       Date:  1976-01-23

5.  Purification and properties of alpha-amylase from chicken (Gallus gallus L.) pancreas.

Authors:  V Buonocore; R Deponte; F Gramenzi; T Petrucci; E Poerio; V Silano
Journal:  Mol Cell Biochem       Date:  1977-08-19       Impact factor: 3.396

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Stability of the conformation of saccharifying bacterial alpha-amylase.

Authors:  A Yutani; K Yutani; T Isemura
Journal:  J Biochem       Date:  1969-02       Impact factor: 3.387

8.  Association of Tenebrio molitor L. alpha-amylase with two protein inhibitors--one monomeric, one dimeric--from wheat flour. Differential scanning calorimetric comparison of heat stabilities.

Authors:  V Silano; J C Zahnley
Journal:  Biochim Biophys Acta       Date:  1978-03-28

9.  Interaction of wheat monomeric and dimeric protein inhibitors with alpha-amylase from yellow mealworm (Tenebrio molitor L. larva).

Authors:  V Buonocore; F Gramenzi; W Pace; T Petrucci; E Poerio; V Silano
Journal:  Biochem J       Date:  1980-06-01       Impact factor: 3.857

10.  Adaptation of the domestic chicken, Gallus domesticus, to continuous feeding of albumin amylase inhibitors from wheat flour as gastro-resistant microgranules.

Authors:  A Macri; R Parlamenti; V Silano; F Valfre
Journal:  Poult Sci       Date:  1977-03       Impact factor: 3.352
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  2 in total

1.  Purification and characterization of novel α-amylase from Bacillus subtilis KIBGE HAS.

Authors:  Saeeda Bano; Shah Ali Ul Qader; Afsheen Aman; Muhammad Noman Syed; Abid Azhar
Journal:  AAPS PharmSciTech       Date:  2011-01-14       Impact factor: 3.246

2.  Amylolytic microorganism from são paulo zoo composting: isolation, identification, and amylase production.

Authors:  Renata C Pascon; Rogério Faria Bergamo; Rafael Xavier Spinelli; Elisangela Dutra de Souza; Diego Magno Assis; Luiz Juliano; Marcelo Afonso Vallim
Journal:  Enzyme Res       Date:  2011-08-09
  2 in total

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