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Literature DB >> 6188215

Bacterial characterization by flow cytometry.

M A Van Dilla, R G Langlois, D Pinkel, D Yajko, W K Hadley.

Abstract

Bacteria were analyzed in a dual-beam flow cytometer after double staining with the fluorescent dyes chromomycin A3 and Hoechst 33258, which bind preferentially to DNA that is rich in guanine-cytosine and adenine-thymine, respectively. The measurements were indicative of the cellular DNA content and base composition, cell concentration, and proliferative state of the population. The ratio of the chromomycin A3 signal to the Hoechst 33258 signal increased with the guanine-cytosine content of the cellular DNA for the six cultured species measured, following expectation. Bacteria in urine from patients with urinary tract infections were characterized without interference from host cell DNA, debris, or other particulates.

Entities: Chemical Disease Species

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Year: 1983 PMID： 6188215 DOI： 10.1126/science.6188215

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

22 in total

1. Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry.

Authors: M V Zubkov; B M Fuchs; H Eilers; P H Burkill; R Amann
Journal: Appl Environ Microbiol Date: 1999-07 Impact factor: 4.792

2. Correlating micromorphology of the pulpal odontoblast seam using confocal laser scanning microscopy and dark field microscopy.

Authors: K A Grötz; B Al-Nawas; H Duschner
Journal: Clin Oral Investig Date: 2003-02-12 Impact factor: 3.573

Review 3. High-speed chromosome sorting.

Authors: Sherrif F Ibrahim; Ger van den Engh
Journal: Chromosome Res Date: 2004 Impact factor: 5.239

9. Gigantism in a bacterium, Epulopiscium fishelsoni, correlates with complex patterns in arrangement, quantity, and segregation of DNA.

Authors: V Bresler; W L Montgomery; L Fishelson; P E Pollak
Journal: J Bacteriol Date: 1998-11 Impact factor: 3.490

10. Bacterium-host cell interactions at the cellular level: fluorescent labeling of bacteria and analysis of short-term bacterium-phagocyte interaction by flow cytometry.

Authors: R B Raybourne; V K Bunning
Journal: Infect Immun Date: 1994-02 Impact factor: 3.441

Bacterial characterization by flow cytometry.

1. Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry.

2. Correlating micromorphology of the pulpal odontoblast seam using confocal laser scanning microscopy and dark field microscopy.

Review 3. High-speed chromosome sorting.

4. Binding of viridans group streptococci to human platelets: a quantitative analysis.

5. Selective detection of bacteria in urine with a long-range surface plasmon waveguide biosensor.

6. Physical characterization and quantification of bacteria by sedimentation field-flow fractionation.

Review 7. Quantifying heterogeneity: flow cytometry of bacterial cultures.

8. Method for flow cytometric detection of Listeria monocytogenes in milk.

9. Gigantism in a bacterium, Epulopiscium fishelsoni, correlates with complex patterns in arrangement, quantity, and segregation of DNA.

10. Bacterium-host cell interactions at the cellular level: fluorescent labeling of bacteria and analysis of short-term bacterium-phagocyte interaction by flow cytometry.