Literature DB >> 6187941

Isolation and partial characterization of a monoclonal antibody to the Rous sarcoma virus transforming protein pp60src.

S J Parsons, D J McCarley, C M Ely, D C Benjamin, J T Parsons.   

Abstract

Transformation of cells by Rous sarcoma virus is mediated by the product of the viral src gene, pp60src. A hybridoma cell line producing an immunoglobulin G3 antibody to pp60src was isolated after lymph node cells from immune mice were fused with mouse myeloma cells (P3-NS1-1). Mice were immunized with p60src purified from Escherichia coli cells expressing the src gene product. The monoclonal antibody immunoprecipitated pp60src from Rous sarcoma virus-transformed cells and recognized an antigenic determinant located in the amino-terminal third of the pp60src protein.

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Year:  1983        PMID： 6187941      PMCID： PMC256532     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  36 in total

1.  The src gene product of transformed and morphologically reverted ASV-infected mammalian cells.

Authors:  M S Collett; J S Brugge; R L Erikson; A F Lau; R A Krzyzek; A J Faras
Journal:  Nature       Date:  1979-09-20       Impact factor: 49.962

2.  Uninfected vertebrate cells contain a protein that is closely related to the product of the avian sarcoma virus transforming gene (src).

Authors:  H Oppermann; A D Levinson; H E Varmus; L Levintow; J M Bishop
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

3.  Characterization of the avian sarcoma virus protein p60src.

Authors:  J S Brugge; P J Steinbaugh; R L Erikson
Journal:  Virology       Date:  1978-11       Impact factor: 3.616

4.  Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.

Authors:  R L Erikson; M S Collett; E Erikson; A F Purchio
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

5.  Src Gene product from different strains of avian sarcoma virus: Kinetics and possible mechanism of heat inactivation of protein kinase activity from cells infected by transformation-defective, temperature-sensitive mutant and wild-type virus.

Authors:  H Rübsamen; R R Friis; H Bauer
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

6.  Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.

Authors:  M S Collett; E Erikson; R L Erikson
Journal:  J Virol       Date:  1979-02       Impact factor: 5.103

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Characterization of a normal avian cell protein related to the avian sarcoma virus transforming gene product.

Authors:  M S Collett; J S Brugge; R L Erikson
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

9.  Temperature-sensitive transformation by Rous sarcoma virus and temperature-sensitive protein kinase activity.

Authors:  B M Sefton; T Hunter; K Beemon
Journal:  J Virol       Date:  1980-01       Impact factor: 5.103

10.  Immune response to the src gene product in mice bearing tumors induced by injection of avian sarcoma virus-transformed mouse cells.

Authors:  S J Parsons; S C Riley; E E Mullen; E J Brock; D C Benjamin; W M Kuehl; J T Parsons
Journal:  J Virol       Date:  1979-10       Impact factor: 5.103
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  11 in total

1.  Inhibition of the tyrosine kinase activity of v-src, v-fgr, and v-yes gene products by a monoclonal antibody which binds both amino and carboxy peptide fragments of pp60v-src.

Authors:  D J McCarley; J T Parsons; D C Benjamin; S J Parsons
Journal:  J Virol       Date:  1987-06       Impact factor: 5.103

2.  Forms of pp60v-src isolated from Rous sarcoma virus-transformed cells.

Authors:  M S Collett; S K Belzer
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

3.  Development and characterization of antisera specific for amino- and carboxy-terminal regions of pp60src.

Authors:  M D Resh; R L Erikson
Journal:  J Virol       Date:  1985-07       Impact factor: 5.103

4.  Localization of conserved and nonconserved epitopes within the Rous sarcoma virus-encoded src protein.

Authors:  S J Parsons; D J McCarley; V W Raymond; J T Parsons
Journal:  J Virol       Date:  1986-09       Impact factor: 5.103

5.  Altered processing of a major secreted phosphoprotein correlates with tumorigenicity in Rous sarcoma virus-transformed mammalian cells.

Authors:  I Chackalaparampil; D Banerjee; Y Poirier; B B Mukherjee
Journal:  J Virol       Date:  1985-03       Impact factor: 5.103

6.  Site-directed point mutation in the src gene oF rous sarcoma virus results in an inactive src gene product.

Authors:  D Bryant; J T Parsons
Journal:  J Virol       Date:  1983-03       Impact factor: 5.103

7.  Amino acid alterations within a highly conserved region of the Rous sarcoma virus src gene product pp60src inactivate tyrosine protein kinase activity.

Authors:  D L Bryant; J T Parsons
Journal:  Mol Cell Biol       Date:  1984-05       Impact factor: 4.272

8.  Isolation of monoclonal antibodies specific for products of avian oncogene myb.

Authors:  G I Evan; G K Lewis; J M Bishop
Journal:  Mol Cell Biol       Date:  1984-12       Impact factor: 4.272

9.  Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.

Authors:  L A Lipsich; A J Lewis; J S Brugge
Journal:  J Virol       Date:  1983-11       Impact factor: 5.103

10.  Rous sarcoma virus variants that encode src proteins with an altered carboxy terminus are defective for cellular transformation.

Authors:  V W Wilkerson; D L Bryant; J T Parsons
Journal:  J Virol       Date:  1985-08       Impact factor: 5.103
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