Literature DB >> 6154714

Vital DNA staining and cell sorting by flow microfluorometry.

M J Lydon, K D Keeler, D B Thomas.   

Abstract

A procedure has been investigated for sorting viable cells according to their DNA content. Cells are stained with the U.V. activated fluorochromes 4'6-diamidino-2-phenylindole (DAPI), Hoechst 33258 or Hoechst 33342, and sorted with a Fluorescence Activated Cell Sorter. Hoechst 33342 is a suitable vital stain for a variety of cell types. Hoechst 33258 and DAPI, however, are quantitative vital stains for CHO cells only. Cloning efficiency is unaffected by the sorting procedure, and these stains are not mutagenic at concentrations suitable for vital staining. Potential applications of this procedure to cell biology are discussed.

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Year:  1980        PMID： 6154714     DOI： 10.1002/jcp.1041020208

Source DB:  PubMed          Journal:  J Cell Physiol        ISSN： 0021-9541            Impact factor:   6.384


  15 in total

1.  Vital DNA staining of agarose-embedded protoplasts and cell suspensions of Nicotiana plumbaginifolia.

Authors:  H C van der Valk; J Blaas; J W van Eck; H A Verhoeven
Journal:  Plant Cell Rep       Date:  1988-12       Impact factor: 4.570

2.  Microscopic and flow cytophotometric analysis of parasitemia in cultures of Plasmodium falciparum vitally stained with Hoechst 33342--application to studies of antimalarial agents.

Authors:  R M Franklin; R Brun; A Grieder
Journal:  Z Parasitenkd       Date:  1986

3.  Induction of Epstein-Barr virus nuclear antigens.

Authors:  D J Moss; T B Sculley; J H Pope
Journal:  J Virol       Date:  1986-06       Impact factor: 5.103

4.  Cell cycle dependence of Hoechst 33342 dye cytotoxicity on sorted living cells.

Authors:  C Wiezorek
Journal:  Histochemistry       Date:  1984

Review 5.  The biosociology of pancreatic B cells.

Authors:  D Pipeleers
Journal:  Diabetologia       Date:  1987-05       Impact factor: 10.122

6.  Visualizing light-triggered release of molecules inside living cells.

Authors:  Ryan Huschka; Oara Neumann; Aoune Barhoumi; Naomi J Halas
Journal:  Nano Lett       Date:  2010-10-13       Impact factor: 11.189

7.  Intracellular morphological changes in Staphylococcus aureus induced by treatment with sodium hypochlorite.

Authors:  Shiori Ujimine; Shigenobu Tone; Mineki Saito; Sakuo Yamada
Journal:  Med Mol Morphol       Date:  2017-05-17       Impact factor: 2.309

8.  Chemosensitivity testing of human gliomas using a fluorescent microcarrier technique.

Authors:  A P Bowles; C G Pantazis; W Wansley; M B Allen
Journal:  J Neurooncol       Date:  1990-04       Impact factor: 4.130

9.  Tracing and ablation of single cells in the mammalian blastocyst using fluorescent DNA staining and multi-photon laser microscopy.

Authors:  Tobias Reupke; Bernd Püschel; Christoph Viebahn
Journal:  Histochem Cell Biol       Date:  2008-12-20       Impact factor: 4.304

10.  Visualization of Endoplasmic Reticulum and Mitochondria in Aurantiochytrium limacinum by the Expression of EGFP with Cell Organelle-Specific Targeting/Retaining Signals.

Authors:  Nozomu Okino; Hiroyoshi Wakisaka; Yohei Ishibashi; Makoto Ito
Journal:  Mar Biotechnol (NY)       Date:  2018-01-29       Impact factor: 3.619
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