Literature DB >> 2422821

Microscopic and flow cytophotometric analysis of parasitemia in cultures of Plasmodium falciparum vitally stained with Hoechst 33342--application to studies of antimalarial agents.

R M Franklin, R Brun, A Grieder.   

Abstract

Conditions for rapid vital staining of Plasmodium falciparum infected human erythrocytes were 1 microgram/ml of the dye Hoechst 33342 for 15 min in the standard culture medium at 37 degrees C. Fixed and stained cultures were analyzed by fluorescence microscopy and flow cytophotometry. The usefulness of this type of analysis for in vitro studies of antimalarial agents was demonstrated using three such agents--cyclosporin A, chloroquine, and pyrimethamine.

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Year:  1986        PMID： 2422821     DOI： 10.1007/bf00931147

Source DB:  PubMed          Journal:  Z Parasitenkd        ISSN： 0044-3255


  19 in total

1.  Babesia rodhaini, Babesia bovis, and Babesia bigemina: analysis and sorting of red cells from infected mouse or calf blood by flow fluorimetry using 33258 Hoechst.

Authors:  R J Howard; B J Rodwell
Journal:  Exp Parasitol       Date:  1979-12       Impact factor: 2.011

2.  Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis.

Authors:  S A Latt; G Stetten
Journal:  J Histochem Cytochem       Date:  1976-01       Impact factor: 2.479

3.  Cytofluorograf detection of Plasmodium yoelii, Trypanosoma gambiense, and Trypanosoma equiperdum by laser excited fluorescence of stained rodent blood.

Authors:  P R Jackson; D G Winkler; S L Kimzey; F M Fisher
Journal:  J Parasitol       Date:  1977-08       Impact factor: 1.276

4.  Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method.

Authors:  J B Jensen; W Trager
Journal:  J Parasitol       Date:  1977-10       Impact factor: 1.276

5.  Analysis and sorting of living cells according to deoxyribonucleic acid content.

Authors:  D J Arndt-Jovin; T M Jovin
Journal:  J Histochem Cytochem       Date:  1977-07       Impact factor: 2.479

6.  Plasmodium berghei - infected red cells sorted according to DNA content.

Authors:  R J Howard; F L Battye
Journal:  Parasitology       Date:  1979-06       Impact factor: 3.234

7.  Antimalarial activity of cyclosporin A.

Authors:  K Thommen-Scott
Journal:  Agents Actions       Date:  1981-12

8.  Synergistic antimalarial activity of pyrimethamine and sulfadoxine against Plasmodium falciparum in vitro.

Authors:  J D Chulay; W M Watkins; D G Sixsmith
Journal:  Am J Trop Med Hyg       Date:  1984-05       Impact factor: 2.345

9.  Staining of Plasmodium yoelii-infected mouse erythrocytes with the fluorescent dye rhodamine 123.

Authors:  K Tanabe
Journal:  J Protozool       Date:  1983-11

10.  Plasmodium-infected blood cells analyzed and sorted by flow fluorimetry with the deoxyribonucleic acid binding dye 33258 Hoechst.

Authors:  R J Howard; F L Battye; G F Mitchell
Journal:  J Histochem Cytochem       Date:  1979-04       Impact factor: 2.479
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  6 in total

1.  Use of radioactive ethanolamine incorporation into phospholipids to assess in vitro antimalarial activity by the semiautomated microdilution technique.

Authors:  N Elabbadi; M L Ancelin; H J Vial
Journal:  Antimicrob Agents Chemother       Date:  1992-01       Impact factor: 5.191

Review 2.  Methodology and application of flow cytometry for investigation of human malaria parasites.

Authors:  Brian T Grimberg
Journal:  J Immunol Methods       Date:  2011-02-04       Impact factor: 2.303

3.  Flow cytometric analysis of Eimeria tenella sporozoite populations exposed to salinomycin sodium in vitro: a comparative study using light and electron microscopy and an in vitro sporozoite invasion-inhibition test.

Authors:  W Raether; H Mehlhorn; J Hofmann; B Bräu; K Ehrlich
Journal:  Parasitol Res       Date:  1991       Impact factor: 2.289

4.  Monitoring Plasmodium falciparum growth and development by UV flow cytometry using an optimized Hoechst-thiazole orange staining strategy.

Authors:  Brian T Grimberg; John J Erickson; R Michael Sramkoski; James W Jacobberger; Peter A Zimmerman
Journal:  Cytometry A       Date:  2008-06       Impact factor: 4.355

5.  Coupling the Antimalarial Cell Penetrating Peptide TP10 to Classical Antimalarial Drugs Primaquine and Chloroquine Produces Strongly Hemolytic Conjugates.

Authors:  Luísa Aguiar; Arnau Biosca; Elena Lantero; Jiri Gut; Nuno Vale; Philip J Rosenthal; Fátima Nogueira; David Andreu; Xavier Fernàndez-Busquets; Paula Gomes
Journal:  Molecules       Date:  2019-12-12       Impact factor: 4.411

6.  Comparison between Flow Cytometry, Microscopy, and Lactate Dehydrogenase-Based Enzyme-Linked Immunosorbent Assay for Plasmodium falciparum Drug Susceptibility Testing under Field Conditions.

Authors:  Charles J Woodrow; Chirapat Wangsing; Kanlaya Sriprawat; Peter R Christensen; Francois Nosten; Laurent Rénia; Bruce Russell; Benoît Malleret
Journal:  J Clin Microbiol       Date:  2015-08-12       Impact factor: 5.948
  6 in total

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