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Literature DB >> 6153398

A rapid method for accurate DNA measurements in single cells in situ using a simple microfluorimeter and Hoechst 33258 as a quantitative fluorochrome.

Abstract

A method is described for the rapid, accurate measurement of single cells in situ using microfluorimetry. This method involves a shutter system, which allows irradiation of single cells for fractions of a second and a peak fluorescence intensity recording device. In this way errors due to fluorochrome fading are almost eliminated and standard deviations of less than 5% are obtained. Hoechst 33258 has been used as a quantitative fluorochrome. Optimum fixation and staining conditions on glass and plastic tissue culture vessels are described.

Entities: Chemical

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Year: 1980 PMID： 6153398 DOI： 10.1177/28.3.6153398

Source DB: PubMed Journal: J Histochem Cytochem ISSN： 0022-1554 Impact factor: 2.479

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Cited

10 in total

1. Observations on enzymatically isolated, living and fixed embryo sacs in several angiosperm species.

Authors: C Zhou; H Y Yang
Journal: Planta Date: 1985-08 Impact factor: 4.116

2. Determination of cell concentration in a plant cell suspension using a fluorescence microplate reader.

Authors: Laurence Lamboursain; Mario Jolicoeur
Journal: Plant Cell Rep Date: 2004-12-10 Impact factor: 4.570

3. Expression of the α-tocopherol transfer protein gene is regulated by oxidative stress and common single-nucleotide polymorphisms.

Authors: Lynn Ulatowski; Cara Dreussi; Noa Noy; Jill Barnholtz-Sloan; Eric Klein; Danny Manor
Journal: Free Radic Biol Med Date: 2012-10-16 Impact factor: 7.376

4. Microfluorometric investigations of chromatin structure. I. Evaluation of nine DNA-specific fluorochromes as probes of chromatin organization.

Authors: R R Cowden; S K Curtis
Journal: Histochemistry Date: 1981

9. Terminal differentiation of ectodermal epithelial stem cells of Hydra can occur in G2 without requiring mitosis or S phase.

Authors: S Dübel; H C Schaller
Journal: J Cell Biol Date: 1990-04 Impact factor: 10.539

10. Inhibition of hepatocellular carcinoma growth by adenovirus-mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice.

Authors: Lei He; Han-Xian Gong; Xiang-Pen Li; Yi-Dong Wang; Yi Li; Jun-Jian Huang; Dan Xie; Hsiang-Fu Kung; Ying Peng
Journal: Oncol Lett Date: 2013-07-16 Impact factor: 2.967

10 in total

A rapid method for accurate DNA measurements in single cells in situ using a simple microfluorimeter and Hoechst 33258 as a quantitative fluorochrome.

1. Observations on enzymatically isolated, living and fixed embryo sacs in several angiosperm species.

2. Determination of cell concentration in a plant cell suspension using a fluorescence microplate reader.

3. Expression of the α-tocopherol transfer protein gene is regulated by oxidative stress and common single-nucleotide polymorphisms.

4. Microfluorometric investigations of chromatin structure. I. Evaluation of nine DNA-specific fluorochromes as probes of chromatin organization.

5. Four fluorochromes for the demonstration and microfluorometric estimation of RNA.

6. DNA distribution of mast cell populations in growing rats.

7. An evaluation of Feulgen-acriflavine-SO2 and Hoechst 33258 for DNA cytofluorometry in tumour pathology.

8. Head activator acts as an autocrine growth factor for NH15-CA2 cells in the G2/mitosis transition.

9. Terminal differentiation of ectodermal epithelial stem cells of Hydra can occur in G2 without requiring mitosis or S phase.

10. Inhibition of hepatocellular carcinoma growth by adenovirus-mediated expression of human telomerase reverse transcriptase COOH-27 terminal polypeptide in mice.