Literature DB >> 6144664

Identification of a glutaminyl-tRNA synthetase mutation Saccharomyces cerevisiae.

A P Mitchell, S W Ludmerer.   

Abstract

Saccharomyces cerevisiae glutaminyl-tRNA synthetase mutants were isolated through systematic screening of tight Gln- derivatives of a leaky glutamine auxotroph. These mutations define a single nuclear gene, GLN4. The gln4-1 mutation is specific for Gln-tRNA synthetase and shows a dosage effect in heterozygous diploids. The wild-type Gln-tRNA synthetase exhibits a Km for glutamine of 25 microM; the gln4-1 mutation increases this value 20-fold. These observations strongly suggest that GLN4 encodes the Gln-tRNA synthetase.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6144664      PMCID： PMC215461          DOI： 10.1128/jb.158.2.530-534.1984

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  9 in total

1.  Function and regulation of aminoacyl-tRNA synthetases in prokaryotic and eukaryotic cells.

Authors:  F C Neihardt; J Parker; W G McKeever
Journal:  Annu Rev Microbiol       Date:  1975       Impact factor: 15.500

Review 2.  Aminoacyl-tRNA synthetases: general features and recognition of transfer RNAs.

Authors:  P R Schimmel; D Söll
Journal:  Annu Rev Biochem       Date:  1979       Impact factor: 23.643

3.  A mutant of yeast with a defective methionyl-tRNA synthetase.

Authors:  C S McLaughlin; L H Hartwell
Journal:  Genetics       Date:  1969-03       Impact factor: 4.562

4.  Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis.

Authors:  L H Hartwell; C S McLaughlin
Journal:  J Bacteriol       Date:  1968-11       Impact factor: 3.490

5.  Mutants of yeast with temperature-sensitive isoleucyl-tRNA synthetases.

Authors:  L H Hartwell; C S McLaughlin
Journal:  Proc Natl Acad Sci U S A       Date:  1968-02       Impact factor: 11.205

6.  Purification and properties of glutamine synthetase from Saccharomyces cerevisiae.

Authors:  A P Mitchell; B Magasanik
Journal:  J Biol Chem       Date:  1983-01-10       Impact factor: 5.157

7.  Absence of involvement of glutamine synthetase and of NAD-linked glutamate dehydrogenase in the nitrogen catabolite repression of arginase and other enzymes in Saccharomyces cerevisiae.

Authors:  E L Dubois; M Grenson
Journal:  Biochem Biophys Res Commun       Date:  1974-09-09       Impact factor: 3.575

8.  Synthesis and activation of asparagine in asparagine auxotrophs of Saccharomyces cerevisiae.

Authors:  F Ramos; J M Wiame
Journal:  Eur J Biochem       Date:  1979-03

9.  Genetics of borrelidin resistant mutants of Saccharomyces cerivisiae and properties of their threonyl-tRNA-synthetase.

Authors:  G Nass; K Poralla
Journal:  Mol Gen Genet       Date:  1976-08-10
  9 in total
  10 in total

1.  Sequence of the GLN1 gene of Saccharomyces cerevisiae: role of the upstream region in regulation of glutamine synthetase expression.

Authors:  P L Minehart; B Magasanik
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490

2.  Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain.

Authors:  P L Minehart; B Magasanik
Journal:  Mol Cell Biol       Date:  1991-12       Impact factor: 4.272

3.  The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases.

Authors:  P W Coschigano; B Magasanik
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272

4.  Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae.

Authors:  I Weygand-Durasevic; D Johnson-Burke; D Söll
Journal:  Nucleic Acids Res       Date:  1987-03-11       Impact factor: 16.971

5.  Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase.

Authors:  L K Pape; A Tzagoloff
Journal:  Nucleic Acids Res       Date:  1985-09-11       Impact factor: 16.971

6.  Molecular characterization of the yeast meiotic regulatory gene RIM1.

Authors:  S S Su; A P Mitchell
Journal:  Nucleic Acids Res       Date:  1993-08-11       Impact factor: 16.971

7.  Physiological and genetic analysis of the carbon regulation of the NAD-dependent glutamate dehydrogenase of Saccharomyces cerevisiae.

Authors:  P W Coschigano; S M Miller; B Magasanik
Journal:  Mol Cell Biol       Date:  1991-09       Impact factor: 4.272

8.  Regulation of glutamine-repressible gene products by the GLN3 function in Saccharomyces cerevisiae.

Authors:  A P Mitchell; B Magasanik
Journal:  Mol Cell Biol       Date:  1984-12       Impact factor: 4.272

9.  Cloning of GLN4: an essential gene that encodes glutaminyl-tRNA synthetase in Saccharomyces cerevisiae.

Authors:  S W Ludmerer; P Schimmel
Journal:  J Bacteriol       Date:  1985-08       Impact factor: 3.490

10.  A yeast glutamine tRNA signals nitrogen status for regulation of dimorphic growth and sporulation.

Authors:  L E Murray; N Rowley; I W Dawes; G C Johnston; R A Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1998-07-21       Impact factor: 11.205
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.