Literature DB >> 6129248

Purification and properties of glutamine synthetase from Saccharomyces cerevisiae.

A P Mitchell, B Magasanik.   

Abstract

We have purified glutamine synthetase over 130-fold from Saccharomyces cerevisiae. The enzyme exhibits a Km for glutamate of 6.3 mM and a Km for ATP of 1.3 mM in the biosynthetic reaction, with a pH optimum from 6.1 to 7.0. Ten to twelve 43,000 molecular weight subunits comprise the active enzyme of 470,000 molecular weight. Rabbit antibodies prepared against the purified enzyme were used to show that induction of enzyme activity correlates with de novo synthesis of the enzyme subunit.

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Year:  1983        PMID： 6129248

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  30 in total

1.  Sequence of the GLN1 gene of Saccharomyces cerevisiae: role of the upstream region in regulation of glutamine synthetase expression.

Authors:  P L Minehart; B Magasanik
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490

2.  Proline metabolism in the wild-type and in a salt-tolerant mutant of nicotiana plumbaginifolia studied by (13)C-nuclear magnetic resonance imaging

Authors: 
Journal:  Plant Physiol       Date:  1999-12       Impact factor: 8.340

3.  Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain.

Authors:  P L Minehart; B Magasanik
Journal:  Mol Cell Biol       Date:  1991-12       Impact factor: 4.272

4.  The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases.

Authors:  P W Coschigano; B Magasanik
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272

5.  The concentration of ammonia regulates nitrogen metabolism in Saccharomyces cerevisiae.

Authors:  E G ter Schure; H H Silljé; A J Verkleij; J Boonstra; C T Verrips
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

6.  Regulation of the nitrogen transfer pathway in the arbuscular mycorrhizal symbiosis: gene characterization and the coordination of expression with nitrogen flux.

Authors:  Chunjie Tian; Beth Kasiborski; Raman Koul; Peter J Lammers; Heike Bücking; Yair Shachar-Hill
Journal:  Plant Physiol       Date:  2010-05-06       Impact factor: 8.340

7.  Three regulatory systems control expression of glutamine synthetase in Saccharomyces cerevisiae at the level of transcription.

Authors:  P M Benjamin; J I Wu; A P Mitchell; B Magasanik
Journal:  Mol Gen Genet       Date:  1989-06

8.  Distinctive properties and expression profiles of glutamine synthetase from a plant symbiotic fungus.

Authors:  Barbara Montanini; Marco Betti; Antonio J Márquez; Raffaella Balestrini; Paola Bonfante; Simone Ottonello
Journal:  Biochem J       Date:  2003-07-15       Impact factor: 3.857

9.  The TOR signaling cascade regulates gene expression in response to nutrients.

Authors:  M E Cardenas; N S Cutler; M C Lorenz; C J Di Como; J Heitman
Journal:  Genes Dev       Date:  1999-12-15       Impact factor: 11.361

10.  Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae.

Authors:  Minoo Shakoury-Elizeh; John Tiedeman; Jared Rashford; Tracey Ferea; Janos Demeter; Emily Garcia; Ronda Rolfes; Patrick O Brown; David Botstein; Caroline C Philpott
Journal:  Mol Biol Cell       Date:  2003-12-10       Impact factor: 4.138
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