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Literature DB >> 6138751

Molecular cloning and nucleotide sequence of a human renin cDNA fragment.

F Soubrier, J J Panthier, P Corvol, F Rougeon.

Abstract

We have studied human renin messenger RNA by hybridization with the mouse submaxillary gland (SMG) renin cDNA probe. The human kidney messenger RNA is about 1.6 kilobase (kb) long, similarly to the mouse SMG renin mRNA. A kidney renin cDNA clone of 1.1 kb length was obtained. A comparison of nucleotide sequences of mouse and human cDNA clones reveals conservation of residues involved in catalytic mechanisms and a potential glycosylation site. The human renin molecular probe allowed us to study renin expression in human chorionic tissue. The chorionic and kidney renin messenger RNAs are similar in length. The Southern blot analysis reveals the presence of a single renin gene in human DNA.

Entities: Gene Species

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Year: 1983 PMID： 6138751 PMCID： PMC326449 DOI： 10.1093/nar/11.20.7181

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

12 in total

Molecular cloning and nucleotide sequence of a human renin cDNA fragment.

1. Angiotensin-forming enzyme in human brain.

2. A general method for isolation of high molecular weight DNA from eukaryotes.

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

4. Renin concentration in human fetal and maternal tissues.

5. Complete amino acid sequence and maturation of the mouse submaxillary gland renin precursor.

6. Amino acid sequence of mouse submaxillary gland renin.

7. Multiple forms of human renin. Purification and characterization.

8. Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain.

9. Molecular cloning of a mouse submaxillary gland renin cDNA fragment.

10. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.

1. Twin-to-twin transfusion syndrome. Role of the fetal renin-angiotensin system.

2. Identification of an enzyme in human kidney that correctly processes prorenin.

Review 3. Biochemistry and pharmacology of the renin-angiotensin system.

4. Structure of the human renin gene.

5. Human renin gene: structure and sequence analysis.

6. Expression of the human renin gene in transgenic mice throughout ontogeny.

7. Detection and localization of renin messenger RNA in human pathologic tissues using in situ hybridization.

8. Sib pair linkage analysis of renin gene haplotypes in human essential hypertension.

9. Close physical linkage of the murine Ren-1 and Ren-2 loci.

10. Human decidua is a major source of renin.