Literature DB >> 6098829

Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.

K Blochlinger, H Diggelmann.   

Abstract

The DNA coding sequence for the hygromycin B phosphotransferase gene was placed under the control of the regulatory sequences of a cloned long terminal repeat of Moloney sarcoma virus. This construction allowed direct selection for hygromycin B resistance after transfection of eucaryotic cell lines not naturally resistant to this antibiotic, thus providing another dominant marker for DNA transfer in eucaryotic cells.

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Year:  1984        PMID： 6098829      PMCID： PMC369308          DOI： 10.1128/mcb.4.12.2929-2931.1984

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  13 in total

1.  Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition.

Authors:  M J Cabañas; D Vázquez; J Modolell
Journal:  Eur J Biochem       Date:  1978-06-01

2.  Phenotypic suppression and misreading Saccharomyces cerevisiae.

Authors:  A Singh; D Ursic; J Davies
Journal:  Nature       Date:  1979-01-11       Impact factor: 49.962

3.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

4.  Initiation of translation at internal AUG codons in mammalian cells.

Authors:  C C Liu; C C Simonsen; A D Levinson
Journal:  Nature       Date:  1984 May 3-9       Impact factor: 49.962

5.  Nucleotide sequence of the genome of a murine sarcoma virus.

Authors:  C Van Beveren; F van Straaten; J A Galleshaw; I M Verma
Journal:  Cell       Date:  1981-11       Impact factor: 41.582

6.  Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin.

Authors:  M Kozak
Journal:  Nucleic Acids Res       Date:  1984-05-11       Impact factor: 16.971

7.  Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes.

Authors:  A González; A Jiménez; D Vázquez; J E Davies; D Schindler
Journal:  Biochim Biophys Acta       Date:  1978-12-21

8.  Number and location of mouse mammary tumor virus proviral DNA in mouse DNA of normal tissue and of mammary tumors.

Authors:  B Groner; N E Hynes
Journal:  J Virol       Date:  1980-03       Impact factor: 5.103

9.  Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.

Authors:  L Gritz; J Davies
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

10.  Molecularly cloned c-mos(rat) is biologically active.

Authors:  F A van der Hoorn; E Hulsebos; A J Berns; H P Bloemers
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  90 in total

1.  Inhibition of endothelial wound repair by dominant negative connexin inhibitors.

Authors:  B R Kwak; M S Pepper; D B Gros; P Meda
Journal:  Mol Biol Cell       Date:  2001-04       Impact factor: 4.138

2.  Protein kinase CK2-dependent regulation of p53 function: evidence that the phosphorylation status of the serine 386 (CK2) site of p53 is constitutive and stable.

Authors:  L McKendrick; D Milne; D Meek
Journal:  Mol Cell Biochem       Date:  1999-01       Impact factor: 3.396

3.  Mutations in the hormone regulatory element of mouse mammary tumor virus differentially affect the response to progestins, androgens, and glucocorticoids.

Authors:  P L Gowland; E Buetti
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

4.  Shuttle vectors conferring hygromycin B resistance to E. coli and to mammalian cells. Differential expression of carboxyterminal fusion proteins.

Authors:  F A Asselbergs; R Pronk
Journal:  Mol Biol Rep       Date:  1992-11       Impact factor: 2.316

5.  In vivo kinetics of mRNA splicing and transport in mammalian cells.

Authors:  A Audibert; D Weil; F Dautry
Journal:  Mol Cell Biol       Date:  2002-10       Impact factor: 4.272

6.  Targeted inactivation of the insulin receptor gene in mouse 3T3-L1 fibroblasts via homologous recombination.

Authors:  D Accili; S I Taylor
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

7.  Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.

Authors:  S Pellegrini; J John; M Shearer; I M Kerr; G R Stark
Journal:  Mol Cell Biol       Date:  1989-11       Impact factor: 4.272

8.  A mouse hybrid cell line that supports gene expression from a variety of promoters in amplifiable vectors.

Authors:  T V Gopal; T Polte; P Arthur; M Seidman
Journal:  In Vitro Cell Dev Biol       Date:  1989-12

9.  Stable expression of antibiotic resistance genes using a promoter fragment of the U1 snRNA gene.

Authors:  F A Asselbergs; R Pronk
Journal:  Mol Biol Rep       Date:  1993-02       Impact factor: 2.316

10.  Glycosylphosphatidylinositol-anchored CD4 supports human immunodeficiency virus type 1 replication, but not cytopathic effect, in T-cell transfectants.

Authors:  W L Marshall; E S Mittler; P Avery; J P Lawrence; R W Finberg
Journal:  J Virol       Date:  1994-06       Impact factor: 5.103
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