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Literature DB >> 6096676

Escherichia coli alpha-haemolysin synthesis and export genes are flanked by a direct repetition of IS91-like elements.

J C Zabala, J M García-Lobo, E Diaz-Aroca, F de la Cruz, J M Ortiz.

Abstract

A revised physical map of the alpha-haemolysin plasmid pHly152 has been constructed. The known position of the hly genes in the restriction map of pHly152 allowed us to locate in it a direct repeat of IS elements flanking the hly genes of pHly152. These elements are IS92L, which is a derivative of the previously characterised element IS91 (1.85 kb) by insertion of a sequence of 1.2 kb, and IS92R, an element related to IS91 by a deletion of 0.7 kb and substitution of a 0.2 kb sequence of IS91 by a 1.2 kb heterologous sequence. IS92L is, in turn, flanked by an inverted repetition of sequences of 1.4 kb. These and previously published data strongly suggest that the hly genes spread at some time in evolution by means of the recombinational activity of IS91-like elements.

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Year: 1984 PMID： 6096676 DOI： 10.1007/bf00327927

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

20 in total

1. Elution of DNA from agarose gels after electrophoresis.

Authors: R Yang; J Lis; R Wu
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

Review 2. Transposable elements in prokaryotes.

Authors: N Kleckner
Journal: Annu Rev Genet Date: 1981 Impact factor: 16.830

3. Determination of the functions of hemolytic plasmid pHly152 of Escherichia coli.

Authors: A Noegel; U Rdest; W Goebel
Journal: J Bacteriol Date: 1981-01 Impact factor: 3.490

4. Relationship between plasmid and chromosomal hemolysin determinants of Escherichia coli.

Authors: D Müller; C Hughes; W Goebel
Journal: J Bacteriol Date: 1983-02 Impact factor: 3.490

5. The transposition frequency of IS1-flanked transposons is a function of their size.

Authors: M Chandler; M Clerget; D J Galas
Journal: J Mol Biol Date: 1982-01-15 Impact factor: 5.469

6. A novel cloning vector for the direct selection of recombinant DNA in E. coli.

Authors: H Hennecke; I Günther; F Binder
Journal: Gene Date: 1982-09 Impact factor: 3.688

7. Hemolysis determinant common to Escherichia coli hemolytic plasmids of different incompatibility groups.

Authors: F de la Cruz; D Müller; J M Ortiz; W Goebel
Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

8. Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1.

Authors: F de la Cruz; J Grinsted
Journal: J Bacteriol Date: 1982-07 Impact factor: 3.490

9. Plasmid cistrons controlling synthesis and excretion of the exotoxin alpha-haemolysin of Escherichia coli.

Authors: A Noegel; U Rdest; W Springer; W Goebel
Journal: Mol Gen Genet Date: 1979-10-01

10. Hemolysis determinant common to Escherichia coli strains of different O serotypes and origins.

Authors: F de la Cruz; J C Zabala; J M Ortiz
Journal: Infect Immun Date: 1983-09 Impact factor: 3.441

21 in total

1. DNA sequence of IS91 and identification of the transposase gene.

Authors: M V Mendiola; Y Jubete; F de la Cruz
Journal: J Bacteriol Date: 1992-02 Impact factor: 3.490

Review 2. ISCR elements: novel gene-capturing systems of the 21st century?

Authors: Mark A Toleman; Peter M Bennett; Timothy R Walsh
Journal: Microbiol Mol Biol Rev Date: 2006-06 Impact factor: 11.056

Review 3. Insertion sequences.

Authors: J Mahillon; M Chandler
Journal: Microbiol Mol Biol Rev Date: 1998-09 Impact factor: 11.056

Escherichia coli alpha-haemolysin synthesis and export genes are flanked by a direct repetition of IS91-like elements.

1. Elution of DNA from agarose gels after electrophoresis.

Review 2. Transposable elements in prokaryotes.

3. Determination of the functions of hemolytic plasmid pHly152 of Escherichia coli.

4. Relationship between plasmid and chromosomal hemolysin determinants of Escherichia coli.

5. The transposition frequency of IS1-flanked transposons is a function of their size.

6. A novel cloning vector for the direct selection of recombinant DNA in E. coli.

7. Hemolysis determinant common to Escherichia coli hemolytic plasmids of different incompatibility groups.

8. Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1.

9. Plasmid cistrons controlling synthesis and excretion of the exotoxin alpha-haemolysin of Escherichia coli.

10. Hemolysis determinant common to Escherichia coli strains of different O serotypes and origins.

1. DNA sequence of IS91 and identification of the transposase gene.

Review 2. ISCR elements: novel gene-capturing systems of the 21st century?

Review 3. Insertion sequences.

4. Structure and mobilization of an ampicillin and gentamicin resistance determinant.

5. Genetical and functional organisation of the Escherichia coli haemolysin determinant 2001.

6. The carboxy-terminal region of haemolysin 2001 is required for secretion of the toxin from Escherichia coli.

7. Transposition of IS91 does not generate a target duplication.

8. Regulation of haemolysin synthesis in E. coli determined by HLY genes of human origin.

9. Extensive homology between the leukotoxin of Pasteurella haemolytica A1 and the alpha-hemolysin of Escherichia coli.

10. Evidence for insertion sequence-mediated spread of the thermostable direct hemolysin gene among Vibrio species.