Literature DB >> 6096670

Molecular analysis of F plasmid pif region specifying abortive infection of T7 phage.

D Cram, A Ray, R Skurray.   

Abstract

We report the molecular cloning of the pif region of the F plasmid and its physical dissection by subcloning and deletion analysis. Examination of the polypeptide products synthesized in maxicells by plasmids carrying defined pif sequences has shown that the region specifies at least two proteins of molecular weights 80,000 and 40,000, the genes for which appear to lie in the same transcriptional unit. In addition, analysis of pif-lacZ fusion plasmids has detected a pif promoter and determined the direction of transcription across the pif region.

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Year:  1984        PMID： 6096670     DOI： 10.1007/bf00327934

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  22 in total

1.  Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

Authors:  R A Skurray; H Nagaishi; A J Clark
Journal:  Proc Natl Acad Sci U S A       Date:  1976-01       Impact factor: 11.205

2.  Plasmid vehicles for direct cloning of Escherichia coli promoters.

Authors:  G An; J D Friesen
Journal:  J Bacteriol       Date:  1979-11       Impact factor: 3.490

3.  Second EcoRI fragment of F capable of self-replication.

Authors:  D Lane; R C Gardner
Journal:  J Bacteriol       Date:  1979-07       Impact factor: 3.490

4.  Physical mapping of genes on the F plasmid of Escherichia coli responsible for inhibition of growth of female-specific bacteriophages.

Authors:  S Palchaudhuri; W K Maas
Journal:  J Bacteriol       Date:  1977-11       Impact factor: 3.490

5.  T7 protein synthesis in F' episome-containing cells: assignment of specific proteins to three translational groups.

Authors:  T G Morrison; D D Blumberg; M H Malamy
Journal:  J Virol       Date:  1974-02       Impact factor: 5.103

6.  T7 translational control mechanisms and their inhibiton by F factors.

Authors:  T G Morrison; M H Malamy
Journal:  Nat New Biol       Date:  1971-05-12

7.  Identification of the pifC gene and its role in negative control of F factor pif gene expression.

Authors:  J F Miller; M H Malamy
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490

8.  Recombinants between bacteriophages T7 and T3 which productively infect F-plasmid-containing strains of Escherichia coli.

Authors:  I J Molineux; P Q Mooney; J L Spence
Journal:  J Virol       Date:  1983-06       Impact factor: 5.103

9.  lambda-Transducing phages carrying transfer genes isolated from an abnormal prophage insertion into the traY gene of F.

Authors:  D A Johnson; N S Willetts
Journal:  Plasmid       Date:  1983-01       Impact factor: 3.466

10.  Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

Authors:  M J Casadaban; S N Cohen
Journal:  J Mol Biol       Date:  1980-04       Impact factor: 5.469
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  12 in total

1.  Expression of gene 1.2 and gene 10 of bacteriophage T7 is lethal to F plasmid-containing Escherichia coli.

Authors:  C K Schmitt; I J Molineux
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

2.  Localization of Separate Genetic Loci for Reduced Sensitivity towards Small Isometric-Headed Bacteriophage sk1 and Prolate-Headed Bacteriophage c2 on pGBK17 from Lactococcus lactis subsp. lactis KR2.

Authors:  L L McKay; M J Bohanon; K M Polzin; P L Rule; K A Baldwin
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

3.  Molecular characterization of a genomic region in a Lactococcus bacteriophage that is involved in its sensitivity to the phage defense mechanism AbiA.

Authors:  P K Dinsmore; T R Klaenhammer
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

4.  Incomplete entry of bacteriophage T7 DNA into F plasmid-containing Escherichia coli.

Authors:  L R García; I J Molineux
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

5.  Cloning and analysis of pif, replication and leading regions of the F plasmid.

Authors:  R Jackson; D Cram; A Ray; D DiBerardino; R Skurray
Journal:  Mol Gen Genet       Date:  1984

6.  Expression and site-directed mutagenesis of the lactococcal abortive phage infection protein AbiK.

Authors:  Louis-Charles Fortier; Julie D Bouchard; Sylvain Moineau
Journal:  J Bacteriol       Date:  2005-06       Impact factor: 3.490

7.  Molecular characterization of a new abortive infection system (AbiU) from Lactococcus lactis LL51-1.

Authors:  G Dai; P Su; G E Allison; B L Geller; P Zhu; W S Kim; N W Dunn
Journal:  Appl Environ Microbiol       Date:  2001-11       Impact factor: 4.792

8.  Both the fipA gene of pKM101 and the pifC gene of F inhibit conjugal transfer of RP1 by an effect on traG.

Authors:  J M Santini; V A Stanisich
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490

9.  Genes 1.2 and 10 of bacteriophages T3 and T7 determine the permeability lesions observed in infected cells of Escherichia coli expressing the F plasmid gene pifA.

Authors:  C K Schmitt; P Kemp; I J Molineux
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490

10.  Streptomycin- and rifampin-resistant mutants of Escherichia coli perturb F exclusion of bacteriophage T7 by affecting synthesis of the F plasmid protein PifA.

Authors:  C K Schmidt; P Kemp; I J Molineux
Journal:  J Bacteriol       Date:  1995-03       Impact factor: 3.490
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