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Literature DB >> 6096669

Cloning and analysis of pif, replication and leading regions of the F plasmid.

R Jackson, D Cram, A Ray, D DiBerardino, R Skurray.

Abstract

We describe the molecular cloning of BglII fragments of the hybrid plasmid pRS5 (pSC101 and EcoRI fragments of F; f7, f5, f3 and f6). The clones isolated were examined for the expression of F-specified replication, incompatibility, mobilization and inhibition of T7 bacteriophage multiplication. Proteins directed by the BglII clones were labelled in Escherichia coli K12 maxicells and analyzed by SDS-polyacrylamide gel electrophoresis. The sizes of previously reported proteins, encoded by the replication, incompatibility and leading regions encompassed by these plasmids have been confirmed in this study. In addition, the results demonstrate that a pif gene, which encodes an 80,000 dalton polypeptide essential for the inhibition T7 phage multiplication, is located on the BglII fragment that spans the junction of EcoRI fragments f7 and f5.

Entities: Chemical Species

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Year: 1984 PMID： 6096669 DOI： 10.1007/bf00327933

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

40 in total

1. Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

Authors: R A Skurray; H Nagaishi; A J Clark
Journal: Proc Natl Acad Sci U S A Date: 1976-01 Impact factor: 11.205

2. Construction and BamHL analysis of chimeric plasmids containing EcoRL DNA fragments of the F sex factor.

Authors: R A Skurray; H Nagaishi; A J Clark
Journal: Plasmid Date: 1978-02 Impact factor: 3.466

Review 3. The conjugation system of F-like plasmids.

Authors: N Willetts; R Skurray
Journal: Annu Rev Genet Date: 1980 Impact factor: 16.830

4. Complementation of replication-deficient deletion derivatives of plasmid mini-F.

Authors: J Ebbers; R Eichenlaub
Journal: J Bacteriol Date: 1981-09 Impact factor: 3.490

5. Two mini-F-encoded proteins are essential for equipartition.

Authors: S Austin; A Wierzbicki
Journal: Plasmid Date: 1983-07 Impact factor: 3.466

6. Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat.

Authors: R C Johnson; J C Yin; W S Reznikoff
Journal: Cell Date: 1982-10 Impact factor: 41.582

7. Cloning and polypeptide analysis of the leading region in F plasmid DNA transfer.

Authors: A Ray; R Skurray
Journal: Plasmid Date: 1983-05 Impact factor: 3.466

8. lambda-Transducing phages carrying transfer genes isolated from an abnormal prophage insertion into the traY gene of F.

Authors: D A Johnson; N S Willetts
Journal: Plasmid Date: 1983-01 Impact factor: 3.466

6. Nucleotide sequence of the leading region adjacent to the origin of transfer on plasmid F and its conservation among conjugative plasmids.

Authors: S Loh; D Cram; R Skurray
Journal: Mol Gen Genet Date: 1989-10

6 in total

Cloning and analysis of pif, replication and leading regions of the F plasmid.

1. Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

2. Construction and BamHL analysis of chimeric plasmids containing EcoRL DNA fragments of the F sex factor.

Review 3. The conjugation system of F-like plasmids.

4. Complementation of replication-deficient deletion derivatives of plasmid mini-F.

5. Two mini-F-encoded proteins are essential for equipartition.

6. Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat.

7. Cloning and polypeptide analysis of the leading region in F plasmid DNA transfer.

8. lambda-Transducing phages carrying transfer genes isolated from an abnormal prophage insertion into the traY gene of F.

9. Polarity of Tn5 insertion mutations in Escherichia coli.

10. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.

1. Nucleotide sequence of the psiA (plasmid SOS inhibition) gene located on the leading region of plasmids F and R6-5.

2. Expression of gene 1.2 and gene 10 of bacteriophage T7 is lethal to F plasmid-containing Escherichia coli.

3. The miniF plasmid C protein: sequence, purification and DNA binding.

Review 4. Analysis of the sequence and gene products of the transfer region of the F sex factor.

5. Molecular analysis of F plasmid pif region specifying abortive infection of T7 phage.

6. Nucleotide sequence of the leading region adjacent to the origin of transfer on plasmid F and its conservation among conjugative plasmids.