Literature DB >> 6096214

Rapid restriction mapping of DNA cloned in lambda phage vectors.

H R Rackwitz, G Zehetner, A M Frischauf, H Lehrach.   

Abstract

A protocol for the rapid restriction mapping of phage lambda clones has been developed. Partial digestion products are selectively labelled at the right or left cohesive lambda DNA termini by hybridisation with [32P]oligonucleotides complementary to the single-stranded cos ends. After gel electrophoresis and autoradiography, the restriction map can be directly determined from the "ladder" of partial digestion products.

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Year:  1984        PMID： 6096214     DOI： 10.1016/0378-1119(84)90120-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  91 in total

1.  Evolution of the mouse H-2K region: a hot spot of mutation associated with genes transcribed in embryos and/or germ cells.

Authors:  Y I Yeom; K Abe; K Artzt
Journal:  Genetics       Date:  1992-03       Impact factor: 4.562

2.  Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

Authors:  H Shizuya; B Birren; U J Kim; V Mancino; T Slepak; Y Tachiiri; M Simon
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

3.  At least three promoters direct expression of the mouse glucocorticoid receptor gene.

Authors:  U Strähle; A Schmidt; G Kelsey; A F Stewart; T J Cole; W Schmid; G Schütz
Journal:  Proc Natl Acad Sci U S A       Date:  1992-08-01       Impact factor: 11.205

4.  Allelic variation within the Emv-15 locus defines genomic sequences closely linked to the agouti locus on mouse chromosome 2.

Authors:  L D Siracusa; L B Russell; N A Jenkins; N G Copeland
Journal:  Genetics       Date:  1987-09       Impact factor: 4.562

5.  Differential methylation of the ornithine carbamoyl transferase gene on active and inactive mouse X chromosomes.

Authors:  L J Mullins; G Veres; C T Caskey; V Chapman
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

6.  Cloning and sequencing of the porcine complement factor B.

Authors:  L J Peelman; A R Van de Weghe; W R Coppieters; A J Van Zeveren; Y H Bouquet
Journal:  Immunogenetics       Date:  1991       Impact factor: 2.846

7.  E-box- and MEF-2-independent muscle-specific expression, positive autoregulation, and cross-activation of the chicken MyoD (CMD1) promoter reveal an indirect regulatory pathway.

Authors:  C A Dechesne; Q Wei; J Eldridge; L Gannoun-Zaki; P Millasseau; L Bougueleret; D Caterina; B M Paterson
Journal:  Mol Cell Biol       Date:  1994-08       Impact factor: 4.272

8.  Exon-intron organization of Xenopus MHC class II beta chain genes.

Authors:  F Kobari; K Sato; B P Shum; S Tochinai; M Katagiri; T Ishibashi; L Du Pasquier; M F Flajnik; M Kasahara
Journal:  Immunogenetics       Date:  1995       Impact factor: 2.846

9.  Genomic organization of human complement protein C8 alpha and further examination of its linkage to C8 beta.

Authors:  G A Michelotti; J V Snider; J M Sodetz
Journal:  Hum Genet       Date:  1995-05       Impact factor: 4.132

10.  Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases.

Authors:  S F Haydock; J A Dowson; N Dhillon; G A Roberts; J Cortes; P F Leadlay
Journal:  Mol Gen Genet       Date:  1991-11
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