Literature DB >> 6095190

Hormonal regulation of transcription of rDNA: use of nucleoside thiotriphosphates to measure initiation in isolated nuclei.

Z Y Zhang, E A Thompson, M R Stallcup.   

Abstract

Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Initiation of pre-rRNA was completely resistant to alpha-amanitin but was inhibited by either actinomycin D or heparin. Experiments with P1798 mouse lymphoma cells indicated that the antiproliferative effects of glucocorticoids on lymphoid cells includes a reduction in the ability of nuclei to initiate pre-rRNA chains.

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Year:  1984        PMID： 6095190      PMCID： PMC320285          DOI： 10.1093/nar/12.21.8115

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors:  T Maniatis; A Jeffrey; H van deSande
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

2.  Characterization of mouse ribosomal gene fragments purified by molecular cloning.

Authors:  N Arnheim
Journal:  Gene       Date:  1979-10       Impact factor: 3.688

3.  Integration and transcription of mouse mammary tumor virus DNA in rat hepatoma cells.

Authors:  G M Ringold; P R Shank; H E Varmus; J Ring; K R Yamamoto
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

4.  Transcription initiation site of rat ribosomal DNA.

Authors:  L I Rothblum; R Reddy; B Cassidy
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

5.  Properties of a cell-culture line derived from lymphosarcoma P1798.

Authors:  E A Thompson
Journal:  Mol Cell Endocrinol       Date:  1980-02       Impact factor: 4.102

6.  Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: assay by molecular hybridization.

Authors:  G Ringold; E Y Lasfargues; J M Bishop; H E Varmus
Journal:  Virology       Date:  1975-05       Impact factor: 3.616

7.  Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.

Authors:  K G Miller; B Sollner-Webb
Journal:  Cell       Date:  1981-11       Impact factor: 41.582

8.  Initiation of transcription of ribosomal deoxyribonucleic acid sequences in isolated nuclei of Physarum polycephalum: studies using nucleoside 5'-[gamma-S]triphosphates and labeled precursors.

Authors:  I Y Sun; E M Johnson; V G Allfrey
Journal:  Biochemistry       Date:  1979-10-16       Impact factor: 3.162

9.  Analysis of RNA initiated in isolated mouse myeloma nuclei using purine nucleoside 5'[gamma-S]triphosphates as affinity probes.

Authors:  M M Smith; A E Reeve; R C Huang
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

10.  Initiation of ribosomal RNA chains in homogenates of oocyte nuclei.

Authors:  R A Hipskind; R H Reeder
Journal:  J Biol Chem       Date:  1980-08-25       Impact factor: 5.157
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  2 in total

1.  Hormonal regulation of transcription of rDNA: glucocorticoid effects upon initiation and elongation in vitro.

Authors:  A H Cavanaugh; E A Thompson
Journal:  Nucleic Acids Res       Date:  1985-05-10       Impact factor: 16.971

2.  Analysis and comparison of the internal promoter, pE, of the ilvGMEDA operons from Escherichia coli K-12 and Salmonella typhimurium.

Authors:  J M Lopes; R P Lawther
Journal:  Nucleic Acids Res       Date:  1986-03-25       Impact factor: 16.971
  2 in total

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