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Literature DB >> 6091061

A plasmid vehicle suitable for the molecular cloning and characterization of mammalian promoters.

M S Featherstone, M A Naujokas, B J Pomerantz, J A Hassell.

Abstract

We have constructed a plasmid, pPSG4, that carries only the coding sequences for polyomavirus (Py) small, middle and truncated large T-antigens. A unique HindIII site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late, adenovirus-2 (Ad-2) major late and herpes simplex virus-1 (HSV-1) thymidine kinase (TK) promoters all confer on pPSG4 the ability to transform rat embryonic fibroblasts with high efficiency. Sequential deletion of the 72 bp repeats, the 21 bp repeats and the TATA box from the SV40 early region in pPSG4 produced a 50, then 30 and then a further 5 to 10-fold decrease in transformation efficiency, respectively. Thus pPSG4 is a convenient vector for the cloning and characterization of mammalian promoters.

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Year: 1984 PMID： 6091061 PMCID： PMC320154 DOI： 10.1093/nar/12.18.7235

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

46 in total

1. Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells.

Authors: N Sonenberg; D Guertin; K A Lee
Journal: Mol Cell Biol Date: 1982-12 Impact factor: 4.272

2. Polyoma virus-specific 55K protein isolated from plasma membrane of productively infected cells is virus-coded and important for cell transformation.

Authors: Y Ito
Journal: Virology Date: 1979-10-15 Impact factor: 3.616

3. Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens.

Authors: E Soeda; J R Arrand; N Smolar; B E Griffin
Journal: Cell Date: 1979-06 Impact factor: 41.582

4. Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus.

Authors: D Kleid; Z Humayun; A Jeffrey; M Ptashne
Journal: Proc Natl Acad Sci U S A Date: 1976-02 Impact factor: 11.205

5. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

Authors: M Wigler; A Pellicer; S Silverstein; R Axel
Journal: Cell Date: 1978-07 Impact factor: 41.582

6. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors: J G Sutcliffe
Journal: Cold Spring Harb Symp Quant Biol Date: 1979

7. In vivo sequence requirements of the SV40 early promotor region.

Authors: C Benoist; P Chambon
Journal: Nature Date: 1981-03-26 Impact factor: 49.962

8. The SV40 early region TATA box is required for accurate in vitro initiation of transcription.

Authors: D J Mathis; P Chambon
Journal: Nature Date: 1981-03-26 Impact factor: 49.962

9. Transformation of rat embryo fibroblasts by cloned polyoma virus DNA fragments containing only part of the early region.

Authors: J A Hassell; W C Topp; D B Rifkin; P E Moreau
Journal: Proc Natl Acad Sci U S A Date: 1980-07 Impact factor: 11.205

10. Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters.

Authors: L E Post; S Mackem; B Roizman
Journal: Cell Date: 1981-05 Impact factor: 41.582

6 in total

A plasmid vehicle suitable for the molecular cloning and characterization of mammalian promoters.

1. Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells.

2. Polyoma virus-specific 55K protein isolated from plasma membrane of productively infected cells is virus-coded and important for cell transformation.

3. Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens.

4. Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus.

5. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

6. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

7. In vivo sequence requirements of the SV40 early promotor region.

8. The SV40 early region TATA box is required for accurate in vitro initiation of transcription.

9. Transformation of rat embryo fibroblasts by cloned polyoma virus DNA fragments containing only part of the early region.

10. Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters.

1. The amino terminus of polyomavirus middle T antigen is required for transformation.

2. The polyomavirus enhancer comprises multiple functional elements.

3. Location of sequences in polyomavirus DNA that are required for early gene expression in vivo and in vitro.

4. Overproduction of polyomavirus middle T antigen in mammalian cells through the use of an adenovirus vector.

5. Construction of a helper-free recombinant adenovirus that expresses polyomavirus large T antigen.

6. Requirements for species-specific papovavirus DNA replication.