Literature DB >> 6087294

The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.

L Singh, K W Jones.   

Abstract

The use of heparin as a simple, effective and cheap substitute for conventional methods of controlling background in hybridization procedures is described and illustrated with reference to the use of DNA probes in filter and in situ hybridization. Possible specific mechanisms for this heparin effect are discussed.

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Year:  1984        PMID： 6087294      PMCID： PMC320019          DOI： 10.1093/nar/12.14.5627

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Multiple steps in DNA recognition by restriction endonuclease from E. coli K.

Authors:  R Yuan; T A Bickle; W Ebbers; C Brack
Journal:  Nature       Date:  1975-08-14       Impact factor: 49.962

2.  A method for the detection of RNA-DNA complexes.

Authors:  A P NYGAARD; B D HALL
Journal:  Biochem Biophys Res Commun       Date:  1963-07-18       Impact factor: 3.575

3.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

4.  A simple, general procedure for purifying restriction endonucleases.

Authors:  T A Bickle; V Pirrotta; R Imber
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

5.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

6.  A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.

Authors:  D Gillespie; S Spiegelman
Journal:  J Mol Biol       Date:  1965-07       Impact factor: 5.469

7.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

8.  Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets.

Authors:  M Bittner; P Kupferer; C F Morris
Journal:  Anal Biochem       Date:  1980-03-01       Impact factor: 3.365

9.  A specific complex between a restriction endonuclease and its DNA substrate.

Authors:  R Yuan; M Meselson
Journal:  Proc Natl Acad Sci U S A       Date:  1970-02       Impact factor: 11.205

10.  Effect of different denaturing agents on the detectability of specific DNA sequences of various base compositions by in situ hybridisation.

Authors:  L Singh; I F Purdom; K W Jones
Journal:  Chromosoma       Date:  1977-04-20       Impact factor: 4.316
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  80 in total

1.  Molecular and evolutionary analysis of the growth-controlling region on the human Y chromosome.

Authors:  Stefan Kirsch; Birgit Weiss; Klaus Zumbach; Gudrun Rappold
Journal:  Hum Genet       Date:  2003-10-25       Impact factor: 4.132

2.  Multiple complex families of endogenous retroviruses are highly conserved in the genus Gallus.

Authors:  M T Boyce-Jacino; K O'Donoghue; A J Faras
Journal:  J Virol       Date:  1992-08       Impact factor: 5.103

3.  Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa.

Authors:  G B Kiss; E Vincze; Z Végh; G Tóth; J Soós
Journal:  Plant Mol Biol       Date:  1990-04       Impact factor: 4.076

4.  Identification of a divergent M protein gene and an M protein-related gene family in Streptococcus pyogenes serotype 49.

Authors:  E J Haanes; P P Cleary
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490

5.  Leukotriene B4 up-regulates IL-6 rather than IL-1 synthesis in human monocytes.

Authors:  P E Poubelle; J Stankova; J Grassi; M Rola-Pleszczynski
Journal:  Agents Actions       Date:  1991-09

6.  Sequence and expression of the Cc gene, a member of the dopa decarboxylase gene cluster of Drosophila: possible translational regulation.

Authors:  D D Eveleth; J L Marsh
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

7.  The sequence of a pea vicilin gene and its expression in transgenic tobacco plants.

Authors:  T J Higgins; E J Newbigin; D Spencer; D J Llewellyn; S Craig
Journal:  Plant Mol Biol       Date:  1988-09       Impact factor: 4.076

8.  srfA is an operon required for surfactin production, competence development, and efficient sporulation in Bacillus subtilis.

Authors:  M M Nakano; R Magnuson; A Myers; J Curry; A D Grossman; P Zuber
Journal:  J Bacteriol       Date:  1991-03       Impact factor: 3.490

9.  Novel human endogenous sequences related to human immunodeficiency virus type 1.

Authors:  M S Horwitz; M T Boyce-Jacino; A J Faras
Journal:  J Virol       Date:  1992-04       Impact factor: 5.103

10.  Fc-receptor and M-protein genes of group A streptococci are products of gene duplication.

Authors:  D G Heath; P P Cleary
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205
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