Literature DB >> 568150

Cellular uptake and metabolism of daunorubicin as determined by high-performance liquid chromatography. Application to L1210 cells.

R Baurain, A Zenebergh, A Trouet.   

Abstract

The metabolism of daunorubicin (DNR) to daunorubicinol and daunomycinone in murine leukaemia cells has been examined by means of high-performance liquid chromatography. A rapid and efficient extraction method has been developed that permits the recovery of the drug and its metabolites from cell homogenates. By means of high-performance liquid chromatography daunorubicinol and daunomycinone have been separated from DNR and the intracellular concentration of the compounds determined. The method developed is very rapid and sensitive, and amounts as small as 30 pg of DNR can be detected. The results indicate that the aldo-keto reductase is not very active in L1210 cells in culture, the main intracellular product found being DNR.

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Year:  1978        PMID： 568150     DOI： 10.1016/s0021-9673(00)92350-1

Source DB:  PubMed          Journal:  J Chromatogr


  14 in total

1.  Intracellular concentrations of anti cancer drugs in leukemic cells in vitro vs in vivo.

Authors:  B Sundman-Engberg; U Tidefelt; J Liliemark; C Paul
Journal:  Cancer Chemother Pharmacol       Date:  1990       Impact factor: 3.333

2.  Anthracyclines and their C-13 alcohol metabolites: growth inhibition and DNA damage following incubation with human tumor cells in culture.

Authors:  M J Kuffel; J M Reid; M M Ames
Journal:  Cancer Chemother Pharmacol       Date:  1992       Impact factor: 3.333

3.  Quantitation of cell-associated doxorubicin by high-performance liquid chromatography after enzymatic desequestration.

Authors:  A Andersen; D J Warren; L Slørdal
Journal:  Cancer Chemother Pharmacol       Date:  1994       Impact factor: 3.333

4.  Determination of daunorubicin, doxorubicin and their fluorescent metabolites by high-pressure liquid chromatography: plasma levels in DBA2 mice.

Authors:  R Baurain; D Deprez-De Campeneere; A Trouet
Journal:  Cancer Chemother Pharmacol       Date:  1979       Impact factor: 3.333

5.  Adriamycin uptake and metabolism in organotypic culture of A549 human adenocarcinoma cells according to the exposure time.

Authors:  S Chevillard; P Vielh; G Bastian; J Coppey
Journal:  J Cancer Res Clin Oncol       Date:  1990       Impact factor: 4.553

6.  Cellular pharmacokinetics of aclacinomycin A in cultured L1210 cells. Comparison with daunorubicin and doxorubicin.

Authors:  A Zenebergh; R Baurain; A Trouet
Journal:  Cancer Chemother Pharmacol       Date:  1982       Impact factor: 3.333

7.  Uptake of free and DNA-bound daunorubicin and doxorubicin into human leukemic cells.

Authors:  C Paul; C Peterson; G Gahrton; D Lockner
Journal:  Cancer Chemother Pharmacol       Date:  1979       Impact factor: 3.333

Review 8.  Anthracycline antitumour agents. A review of physicochemical, analytical and stability properties.

Authors:  J Bouma; J H Beijnen; A Bult; W J Underberg
Journal:  Pharm Weekbl Sci       Date:  1986-04-25

9.  Pharmacokinetics of daunorubicin after administration as free drug or as DNA complex in leukemic patients.

Authors:  S O Nilsson; B Andersson; S Eksborg; M Beran; H Ehrsson
Journal:  Cancer Chemother Pharmacol       Date:  1981       Impact factor: 3.333

10.  Hepatic extraction, metabolism and biliary excretion of doxorubicin in the isolated perfused rat liver.

Authors:  F Ballet; P Vrignaud; J Robert; C Rey; R Poupon
Journal:  Cancer Chemother Pharmacol       Date:  1987       Impact factor: 3.333
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