Literature DB >> 5675512

Production of highly labeled adenoviruses.

J P Burnett, A O Summers, J A Harrington, A C Dwyer.   

Abstract

A method is described for increasing the incorporation of radioactive thymidine into adenovirus deoxyribonucleic acid by the use of amethopterin. In addition, a modified procedure is presented for the preparation of highly purified adenoviruses. This procedure, which employs enzymatic digestion of cellular debris, obviates the necessity for fluorocarbon treatment of crude virus suspensions, and routinely provides excellent recovery of virus.

Entities:  

Mesh：

Substances：

Year:  1968        PMID： 5675512      PMCID： PMC547627          DOI： 10.1128/am.16.8.1245-1250.1968

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  12 in total

1.  Amino acid metabolism in mammalian cell cultures.

Authors:  H EAGLE
Journal:  Science       Date:  1959-08-21       Impact factor: 47.728

Review 2.  THE MECHANISM OF ACTION OF THE FOLATE ANTAGONISTS IN MAN.

Authors:  J R BERTINO
Journal:  Cancer Res       Date:  1963-09       Impact factor: 12.701

3.  Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus.

Authors:  M GREEN; M PINA
Journal:  Virology       Date:  1963-05       Impact factor: 3.616

4.  Biochemical studies on adenovirus multiplication. III. Requirement for DNA synthesis.

Authors:  M GREEN
Journal:  Virology       Date:  1962-12       Impact factor: 3.616

5.  Prevention of toxicity of amethopterin for sarcoma-180 cells in tissue culture.

Authors:  M T HAKALA
Journal:  Science       Date:  1957-08-09       Impact factor: 47.728

6.  Studies in histochemistry. I. Determination of nucleic acids in microgram amounts of tissue.

Authors:  J F SCOTT; A P FRACCASTORO; E B TAFT
Journal:  J Histochem Cytochem       Date:  1956-01       Impact factor: 2.479

7.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

8.  On the production of highly labeled tumor virus deoxyribonucleic acid.

Authors:  S W Luborsky; D Lorenz; D Reynolds; J E Verna; E Usdin; P T Mora
Journal:  Proc Natl Acad Sci U S A       Date:  1967-05       Impact factor: 11.205

9.  Antigens and structure of the adenovirus.

Authors:  R C Valentine; H G Pereira
Journal:  J Mol Biol       Date:  1965-08       Impact factor: 5.469

10.  Thymidine kinase activity in synchronized HeLa cell cultures.

Authors:  E Stubblefield; G C Mueller
Journal:  Biochem Biophys Res Commun       Date:  1965-08-16       Impact factor: 3.575
View more
  12 in total

1.  Uncoating and gene expression of simian virus 40 in CV-1 cell nuclei inoculated by microinjection.

Authors:  E G Diacumakos; E L Gershey
Journal:  J Virol       Date:  1977-12       Impact factor: 5.103

2.  Virus particle content of smallpox vaccines.

Authors:  R C Dunlap
Journal:  Appl Microbiol       Date:  1970-04

3.  Mechanisms of photodynamic inactivation of herpes simplex viruses: comparison between methylene blue, light plus electricity, and hematoporhyrin plus light.

Authors:  L E Schnipper; A A Lewin; M Swartz; C S Crumpacker
Journal:  J Clin Invest       Date:  1980-02       Impact factor: 14.808

4.  Evidence for a transcription-control region of Simian virus 40 in the adenovirus 2--Simian virus 40 hybrid, Ad2+ND 1 .

Authors:  C T Patch; A M Lewis; A S Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1972-11       Impact factor: 11.205

5.  Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VII. Characterization of the simian virus 40 RNA species induced by five nondefective hybrid viruses.

Authors:  A S Levine; M J Levin; M N Oxman; A M Lewis
Journal:  J Virol       Date:  1973-05       Impact factor: 5.103

6.  Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties.

Authors:  A M Lewis; A S Levine; C S Crumpacker; M J Levin; R J Samaha; P H Henry
Journal:  J Virol       Date:  1973-05       Impact factor: 5.103

7.  Control of simian virus 40 gene expression in adenovirus-simian virus 40 hybrid viruses. Synthesis of hybrid adenovirus 2-simian virus 40 RNA molecules in cells infected with a nondefective adenovirus 2-simian virus 40 hybrid virus.

Authors:  M N Oxman; M J Levin; A M Lewis
Journal:  J Virol       Date:  1974-02       Impact factor: 5.103

8.  Adenovirus type 2-simian virus 40 hybrid population: evidence for a hybrid deoxyribonucleic acid molecule and the absence of adenovirus-encapsidated circular simian virus 40 deoxyribonucleic acid.

Authors:  C S Crumpacker; M J Levin; W H Wiese; W P Rowe; A M Lewis
Journal:  J Virol       Date:  1970-12       Impact factor: 5.103

9.  Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IV. Characterization of the simian virus 40 ribonucleic acid species induced by wild-type simian virus 40 and by the nondefective hybrid virus, Ad2 + ND 1 .

Authors:  M N Oxman; A S Levine; C S Crumpacker; M J Levin; P H Henry; A M Lewis
Journal:  J Virol       Date:  1971-08       Impact factor: 5.103

10.  Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. II. Relationship of adenovirus 2 deoxyribonucleic acid and simian virus 40 deoxyribonucleic acid in the Ad2+ND genome.

Authors:  M J Levin; C S Crumpacker; A M Lewis; M N Oxman; P H Henry; W P Rowe
Journal:  J Virol       Date:  1971-03       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.