Literature DB >> 566713

Time and cell systems as variables in fusion experiments with polyethylene glycol.

G J Anders, J Wierda, A J Nienhaus, V J Idenburg.   

Abstract

Cell hybridization was done between a monolayer of B14-150 Chinese hamster cells and a suspension of either mouse leukemia cells or normal human lymphocytes. Cell contact was obtained by centrifugation of the suspension cells onto the monolayer cells in a culture plate. Cell fusion was done by means of polyethylene glycol (PEG). The optimum time for PEG exposure as well as the yield of hybrid cells differed markedly with the different combinations.

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Year:  1978        PMID: 566713     DOI: 10.1007/bf00291313

Source DB:  PubMed          Journal:  Hum Genet        ISSN: 0340-6717            Impact factor:   4.132


  12 in total

1.  SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.

Authors:  J W LITTLEFIELD
Journal:  Science       Date:  1964-08-14       Impact factor: 47.728

2.  Mouse lymphoma cells with different radiosensitivities.

Authors:  P ALEXANDER
Journal:  Nature       Date:  1961-11-11       Impact factor: 49.962

3.  New technique for distinguishing between human chromosomes.

Authors:  A T Sumner; H J Evans; R A Buckland
Journal:  Nat New Biol       Date:  1971-07-07

4.  Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro.

Authors:  E H Chu; P Brimer; K B Jacobson; E V Merriam
Journal:  Genetics       Date:  1969-06       Impact factor: 4.562

5.  A method for analysis of chromosomes in hybrid cells employing sequential G-banding and mouse specific D-banding.

Authors:  C J Marshall
Journal:  Exp Cell Res       Date:  1975-03-15       Impact factor: 3.905

6.  A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.

Authors:  W C Russell; C Newman; D H Williamson
Journal:  Nature       Date:  1975-02-06       Impact factor: 49.962

7.  Dimethyl sulfoxide enhances polyethylene glycol-mediated somatic cell fusion.

Authors:  T H Norwood; C J Zeigler; G M Martin
Journal:  Somatic Cell Genet       Date:  1976-05

8.  Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol.

Authors:  R L Davidson; P S Gerald
Journal:  Somatic Cell Genet       Date:  1976-03

9.  Polyethylene glycol-induced mammalian cell hybridization: effect of polyethylene glycol molecular weight and concentration.

Authors:  R L Davidson; K A O'Malley; T B Wheeler
Journal:  Somatic Cell Genet       Date:  1976-05

10.  Time and mode of fusion of human fibroblasts treated with polyethylene glycol (PEG).

Authors:  G Pontecorvo; P N Riddle; A Hales
Journal:  Nature       Date:  1977-01-20       Impact factor: 49.962

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