Literature DB >> 1028174

Polyethylene glycol-induced mammalian cell hybridization: effect of polyethylene glycol molecular weight and concentration.

R L Davidson, K A O'Malley, T B Wheeler.   

Abstract

The effects of polyethylene glycol (PEG) molecular weight and concentration on mammalian cell hybridization were studied. The peak hybridization-inducing activity with all grades of PEG from 400-6000 was found to occur in the concentration range of 50-55%. However, changes in concentration were seen to have different quantitative effects with different grades of PEG. For monolayer fusions, PEG 1000 at 50% seems to be the optimal combination of PEG molecular weight and concentration, in terms of both efficiency of hybridization and relative insensitivity to dilution effects.

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Year:  1976        PMID： 1028174     DOI： 10.1007/BF01538965

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  74 in total

1.  Parameters governing bacterial regeneration and genetic recombination after fusion of Bacillus subtilis protoplasts.

Authors:  M H Gabor; R D Hotchkiss
Journal:  J Bacteriol       Date:  1979-03       Impact factor: 3.490

2.  Polyomavirus genome and polyomavirus enhancer-driven gene expression during myogenesis.

Authors:  R Maione; A Felsani; L Pozzi; M Caruso; P Amati
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

3.  Dissociation of cytotoxicity and DNA cleavage activity induced by topoisomerase II-reactive intercalating agents in hamster-human somatic cell hybrids.

Authors:  B S Glisson; A M Killary; P Merta; W E Ross; J Siciliano; M J Siciliano
Journal:  Cancer Chemother Pharmacol       Date:  1992       Impact factor: 3.333

4.  Purification and properties of oestradiol 17 beta-dehydrogenase extracted from cytoplasmic vesicles of porcine endometrial cells.

Authors:  J Adamski; B Husen; F Marks; P W Jungblut
Journal:  Biochem J       Date:  1992-12-01       Impact factor: 3.857

5.  Another chromosomal assignment for a simian virus 40 integration site in human cells.

Authors:  R Kucherlapati; S P Hwang; N Shimizu; J K McDougall; M R Botchan
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

6.  Accurate modification of a chromosomal plasmid by homologous recombination in human cells.

Authors:  K Y Song; F Schwartz; N Maeda; O Smithies; R Kucherlapati
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

7.  Exaggerated triglyceride accretion in human preadipocyte-murine renal line hybrids composed of cells from massively obese subjects.

Authors:  P E Le Blanc; D A Roncari; D I Hoar; A M Adachi
Journal:  J Clin Invest       Date:  1988-05       Impact factor: 14.808

8.  Stable hybrid myotubes: a new model for studying re-expression of enzymatic activities in vitro.

Authors:  G Meola; V Sansone; G Rotondo; S Radice; G Bottiroli; G Scarlato
Journal:  Ital J Neurol Sci       Date:  1993-01

9.  Spontaneous and restriction enzyme-induced chromosomal recombination in mammalian cells.

Authors:  A R Godwin; R J Bollag; D M Christie; R M Liskay
Journal:  Proc Natl Acad Sci U S A       Date:  1994-12-20       Impact factor: 11.205

10.  Haploid genome reactivation and recovery by cell hybridization. Induction of DNA synthesis in spermatid nuclei.

Authors:  S M Elsevier; F H Ruddle
Journal:  Chromosoma       Date:  1976-07-08       Impact factor: 4.316
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