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Literature DB >> 5642466

The physical state of hemoglobin in sickle-cell anemia erythrocytes in vivo.

Abstract

Venous blood removed anaerobically from patients with sickle-cell anemia was transferred immediately into fixative, thus precluding significant loss or gain of oxygen by the cells. Electron microscopy demonstrated an intraerythrocytic fibrillar fine structure similar to that described in prior studies on erythrocytes sickled by deoxygenation in vitro. Observations reported here lead to these conclusions: (a) explanations of the sickling process derived from in vitro experimentation may with validity be applied to sickling in vivo; and (b) the term "sickled" must be used with caution: a sickle-shaped membrane does not necessarily endose Hb S in filamentous form.

Entities: Chemical Disease Species

Mesh：

Substances：
Hemoglobins

Year: 1968 PMID： 5642466 PMCID： PMC2138473 DOI： 10.1084/jem.127.4.711

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

10 in total

1. [Sickling of erythrocytes studied with polarized light & electron microscopes. II. Erythrocyte internal structure; comparison with intra-erythrocytic crystals].

Authors: M BESSIS; G NOMARSKI; J P THIERY; J BRETON-GORIUS
Journal: Rev Hematol Date: 1958 Apr-Jun

2. Studies on the destruction of red blood cells; irreversibly sickled erythrocytes; their experimental production in vitro.

Authors: S C SHEN; E M FLEMING; W B CASTLE
Journal: Blood Date: 1949-05 Impact factor: 22.113

3. Studies on the destruction of red blood cells. VIII. Molecular orientation in sickle cell hemoglobin solutions.

Authors: J W HARRIS
Journal: Proc Soc Exp Biol Med Date: 1950-10

4. Sickle cell anemia a molecular disease.

Authors: L PAULING; H A ITANO
Journal: Science Date: 1949-11-25 Impact factor: 47.728

5. Molecular mechanism of red cell "sickling".

Authors: M Murayama
Journal: Science Date: 1966-07-08 Impact factor: 47.728

6. Autophagic vacuoles in human red cells.

Authors: G Kent; O T Minick; F I Volini; E Orfei
Journal: Am J Pathol Date: 1966-05 Impact factor: 4.307

7. A modified procedure for lead staining of thin sections.

Authors: G MILLONIG
Journal: J Biophys Biochem Cytol Date: 1961-12

8. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

Authors: E S REYNOLDS
Journal: J Cell Biol Date: 1963-04 Impact factor: 10.539

9. Improvements in epoxy resin embedding methods.

Authors: J H LUFT
Journal: J Biophys Biochem Cytol Date: 1961-02

10. The state of hemoglobin in sickled erythrocytes.

Authors: C A Stetson
Journal: J Exp Med Date: 1966-02-01 Impact factor: 14.307

10 in total

8 in total

1. Structure of sickled erythrocytes and of sickle-cell hemoglobin fibers.

Authors: J T Finch; M F Perutz; J F Bertles; J Döbler
Journal: Proc Natl Acad Sci U S A Date: 1973-03 Impact factor: 11.205

2. Structure of hemoglobin S fibers: optical determination of the molecular orientation in sickled erythrocytes.

Authors: J Hofrichter; D G Hendricker; W A Eaton
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

3. The fine structure of cell-free sickled hemoglobin.

Authors: J G White; B Heagan
Journal: Am J Pathol Date: 1970-01 Impact factor: 4.307

4. Irreversibly sickled erythrocytes: a consequence of the heterogeneous distribution of hemoglobin types in sickle-cell anemia.

Authors: J F Bertles; P F Milner
Journal: J Clin Invest Date: 1968-08 Impact factor: 14.808