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Literature DB >> 5584004

A method for the selective extraction of histone fractions f2(a)1 and f2(a)2 from calf thymus deoxyribonucleoprotein at pH7.

Abstract

1. A new method has been developed for the specific extraction of histone fraction f2(a) from calf thymus deoxyribonucleoprotein at pH7 by using a mixture of ethanol and guanidinium chloride. 2. Fraction f2(a) has been separated into the subfractions f2(a)1 and f2(a)2 by acetone precipitation from acid solution, and at pH7. 3. Modifications of existing electrophoretic methods are described that enable these fractions to be more easily characterized.

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Year: 1967 PMID： 5584004 PMCID： PMC1198352 DOI： 10.1042/bj1050611

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

16 in total

1. The electrophoresis of histones and histone fractions on starch gel.

Authors: E W JOHNS; D M PHILLIPS; P SIMSON; J A BUTLER
Journal: Biochem J Date: 1961-07 Impact factor: 3.857

2. Further fractionations of histones from calf thymus.

Authors: E W JOHNS; J A BUTLER
Journal: Biochem J Date: 1962-01 Impact factor: 3.857

3. The N-terminal groups of calf-thymus histones.

Authors: D M PHILLIPS
Journal: Biochem J Date: 1958-01 Impact factor: 3.857

4. The fractionation and composition of histones from thymus nucleoprotein.

Authors: P F DAVISON; J A BUTLER
Journal: Biochim Biophys Acta Date: 1954-11

Review 5. Correlation of amino-acid composition with certain characteristics of proteins.

Authors: F T Hatch
Journal: Nature Date: 1965-05-22 Impact factor: 49.962

6. Studies on histones. 7. Preparative methods for histone fractions from calf thymus.

Authors: E W Johns
Journal: Biochem J Date: 1964-07 Impact factor: 3.857

7. Interactions of calf-thymus histone fractions in aqueous solution with 8-anilinonaphthalene-1-sulphonic acid.

Authors: D J Laurence
Journal: Biochem J Date: 1966-05 Impact factor: 3.857

8. The acid extraction of histones from calf thymus deoxyribonucleoprotein.

Authors: K Murray
Journal: J Mol Biol Date: 1966-02 Impact factor: 5.469

9. Stepwise removal of protein from a deoxyribonucleoprotein complex and de-repression of the genome.

Authors: G P Georgiev; L N Ananieva; J V Kozlov
Journal: J Mol Biol Date: 1966-12-28 Impact factor: 5.469

10. The electrophoresis of histones in polyacrylamide gel and their quantitative determination.

Authors: E W Johns
Journal: Biochem J Date: 1967-07 Impact factor: 3.857

20 in total

1. The structure and properties of histone F2a comprising the heterologous group F2a1 and F2a2 studied by 13C nuclear magnetic resonance.

Authors: V M Clark; D M Lilley; O W Howarth; B M Richards; J F Pardon
Journal: Nucleic Acids Res Date: 1974-07 Impact factor: 16.971

10. Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications.

Authors: Pedro Rodriguez-Collazo; Sanford H Leuba; Jordanka Zlatanova
Journal: Nucleic Acids Res Date: 2009-05-13 Impact factor: 16.971

A method for the selective extraction of histone fractions f2(a)1 and f2(a)2 from calf thymus deoxyribonucleoprotein at pH7.

1. The electrophoresis of histones and histone fractions on starch gel.

2. Further fractionations of histones from calf thymus.

3. The N-terminal groups of calf-thymus histones.

4. The fractionation and composition of histones from thymus nucleoprotein.

Review 5. Correlation of amino-acid composition with certain characteristics of proteins.

6. Studies on histones. 7. Preparative methods for histone fractions from calf thymus.

7. Interactions of calf-thymus histone fractions in aqueous solution with 8-anilinonaphthalene-1-sulphonic acid.

8. The acid extraction of histones from calf thymus deoxyribonucleoprotein.

9. Stepwise removal of protein from a deoxyribonucleoprotein complex and de-repression of the genome.

10. The electrophoresis of histones in polyacrylamide gel and their quantitative determination.

1. The structure and properties of histone F2a comprising the heterologous group F2a1 and F2a2 studied by 13C nuclear magnetic resonance.

2. A sedimentation study of the interaction of superhelical SV40 DNA with H1 histone.

3. Homologies in amino acid composition and structure of histone F2al isolated from human leukaemic cells.

4. Interactions between the lysine-rich histone F1 and deoxyribonucleic acid.

5. N-Terminal acetyl-peptides from two calf thymus histones.

6. The incorporation of L-(3H)lysine into the histone fractions of rat thymus in vivo.

7. Changes in the composition of acid extracts of purified Echinus nuclei during cleavage stages and from early embryos.

8. Changing patterns of histone acetylation and RNA synthesis in regeneration of the liver.

9. Histone acetylation and hormone action. Early effects of oestradiol-17beta on histone acetylation in rat uterus.

10. Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications.