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Literature DB >> 6035526

The electrophoresis of histones in polyacrylamide gel and their quantitative determination.

Abstract

1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3. A method has been developed for the quantitative determination of the separated histone fractions by measuring the colour yields of dye-histone complexes formed in the gel.

Entities: Chemical Species

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Year: 1967 PMID： 6035526 PMCID： PMC1270547 DOI： 10.1042/bj1040078

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

16 in total

1. The presence of acetyl groups of histones.

Authors: D M PHILLIPS
Journal: Biochem J Date: 1963-05 Impact factor: 3.857

2. Observations on the species and tissue specificity of histones.

Authors: L HNILICA; E W JOHNS; J A BUTLER
Journal: Biochem J Date: 1962-01 Impact factor: 3.857

3. The electrophoresis of histones and histone fractions on starch gel.

Authors: E W JOHNS; D M PHILLIPS; P SIMSON; J A BUTLER
Journal: Biochem J Date: 1961-07 Impact factor: 3.857

4. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors: R A REISFELD; U J LEWIS; D E WILLIAMS
Journal: Nature Date: 1962-07-21 Impact factor: 49.962

5. On the complexity of calf thymus histone.

Authors: P S RASMUSSEN; K MURRAY; J M LUCK
Journal: Biochemistry Date: 1962-01 Impact factor: 3.162

6. Studies on nuclear proteins. II. Quantitative distribution of histone fractions in various tissues.

Authors: L S Hnilica; L J Edwards; A E Hey
Journal: Biochim Biophys Acta Date: 1966-07-27

7. Studies on histones. 7. Preparative methods for histone fractions from calf thymus.

Authors: E W Johns
Journal: Biochem J Date: 1964-07 Impact factor: 3.857

8. Some aspects of the use of "continuous" and "discontinuous" buffer systems in polyacrylamide gel electrophoresis.

Authors: S Hjertén; S Jerstedt; A Tiselius
Journal: Anal Biochem Date: 1965-05 Impact factor: 3.365

9. Countercurrent-distribution studies on histones.

Authors: J A Butler; D F Power; J Palau
Journal: Biochem J Date: 1967-02 Impact factor: 3.857

10. High-resolution disc electrophoresis of histones. I. An improved method.

Authors: G R Shepherd; L R Gurley
Journal: Anal Biochem Date: 1966-03 Impact factor: 3.365

32 in total

1. The structure and properties of histone F2a comprising the heterologous group F2a1 and F2a2 studied by 13C nuclear magnetic resonance.

Authors: V M Clark; D M Lilley; O W Howarth; B M Richards; J F Pardon
Journal: Nucleic Acids Res Date: 1974-07 Impact factor: 16.971

2. Role of oxygen on growth rate and gene activity in cultured chick-embryo heart cells.

Authors: C Clo'; G C Orlandini; C Guarnieri; C M Caldarera
Journal: Biochem J Date: 1976-01-15 Impact factor: 3.857

3. [Structure and function of chromosomes].

Authors: J Sonnenbichler
Journal: Naturwissenschaften Date: 1974-07

4. In vitro complementation as an assay for new proteins required for bacteriophage T4 DNA replication: purification of the complex specified by T4 genes 44 and 62.

Authors: J Barry; B Alberts
Journal: Proc Natl Acad Sci U S A Date: 1972-09 Impact factor: 11.205

5. The isolation and properties of experimental allergic encephalitogenic protein.

Authors: F B Palmer; R M Dawson
Journal: Biochem J Date: 1969-03 Impact factor: 3.857

6. Histones from the red alga, Rhodymenia palmata?

Authors: J H Duffus; C S Penman; N W Webb
Journal: Experientia Date: 1973-05-15

7. A quantitative study of histones in meiocytes. I. Investigation of the histone amount in cricket spermatogenesis by interference microscopy.

Authors: N A Liapunova; D P Babadjanian
Journal: Chromosoma Date: 1973 Impact factor: 4.316