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Literature DB >> 5472154

A radiochemical assay for glutamine synthetase, and activity of the enzyme in rat tissues.

Abstract

1. A radiochemical assay for glutamine synthetase has been developed in which an ATP-regenerating system is incorporated to prevent accumulation of inhibitory amounts of ADP. It is particularly suitable for assay of the enzyme in crude tissue extracts containing high adenosine triphosphatase activity. 2. A survey of the distribution of the enzyme in tissues from normal male rats showed that activity is present in liver, brain cortex, kidney cortex, spleen, testis and retina. 3. The K(m) of the enzyme for l-glutamate is approx. 1.5x10(-2)m.

Entities: Chemical Disease Gene Species

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Year: 1970 PMID： 5472154 PMCID： PMC1179075 DOI： 10.1042/bj1180035

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

13 in total

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Journal: Biochim Biophys Acta Date: 1963-11-08

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Journal: Biochem J Date: 1955-04 Impact factor: 3.857

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Journal: Arch Biochem Biophys Date: 1957-05 Impact factor: 4.013

5. Kidney glutaminases. III. Glutamine synthesis in the guinea pig kidney.

Authors: R RICHTERICH-VAN BAERLE; L GOLDSTEIN; E H DEARBORN
Journal: Enzymologia Date: 1957-07-31

6. Isolation of glutamine synthetase and glutamotransferase from green peas.

Authors: W H ELLIOTT
Journal: J Biol Chem Date: 1953-04 Impact factor: 5.157

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Authors: V PAMILJANS; P R KRISHNASWAMY; G DUMVILLE; A MEISTER
Journal: Biochemistry Date: 1962-01 Impact factor: 3.162

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Authors: W H ELLIOTT
Journal: Biochem J Date: 1951-06 Impact factor: 3.857

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Authors: P Lund; L Goldstein
Journal: Comp Biochem Physiol Date: 1969-10-15

10. A two-dimensional separation of acid, neutral and basic amino acids by thin layer chromatography on cellulose.

Authors: E Bujard; J Mauron
Journal: J Chromatogr Date: 1966-01

11 in total

1. Distribution of glutamine synthetase in the snapper (Pagrus auratus) and implications for the immune system.

Authors: S P Walker; D Keast; S McBride
Journal: Fish Physiol Biochem Date: 1996-06 Impact factor: 2.794

2. Effects of chronic metabolic acidosis on glutamine turnover and urea production in the perfused rat liver.

Authors: D M Kinlen; E Bourke
Journal: Ir J Med Sci Date: 1975-12 Impact factor: 1.568

3. Simultaneous isolation of glial and neuronal fractions from rat brain homogenates: comparison of high-affinity L-glutamate transport properties.

Authors: K K Daniels; T W Vickroy
Journal: Neurochem Res Date: 1998-01 Impact factor: 3.996

4. On the activity of glutamine synthetase in the red blood cells.

Authors: L Ababei; S Porumb
Journal: Blut Date: 1973-05

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Authors: P A King; L Goldstein; E A Newsholme
Journal: Biochem J Date: 1983-11-15 Impact factor: 3.857

6. Glutamic acid as a precursor to N-terminal pyroglutamic acid in mouse plasmacytoma protein (protein synthesis-initiation-immunoglobulins-pyrrolidone carboxylic acid).

Authors: D R Twardzik; A Peterkofsky
Journal: Proc Natl Acad Sci U S A Date: 1972-01 Impact factor: 11.205

7. Control of glutamine synthesis in rat liver.

Authors: P Lund
Journal: Biochem J Date: 1971-09 Impact factor: 3.857

8. Effects of fasting and diabetes on some enzymes and transport of glutamate in cortex slices or synaptosomes from rat brain.

Authors: E Galanopoulos; V Lellos; M Papadakis; H Philippidis; G Palaiologos
Journal: Neurochem Res Date: 1988-03 Impact factor: 3.996

Review 9. Interorgan ammonia trafficking in liver disease.

Authors: Steven W M Olde Damink; Rajiv Jalan; Cornelius H C Dejong
Journal: Metab Brain Dis Date: 2008-12-09 Impact factor: 3.584

10. Glutamine-synthesizing activity in lungs of fed, starved, acidotic, diabetic, injured and septic rats.

Authors: M S Ardawi
Journal: Biochem J Date: 1990-09-15 Impact factor: 3.857