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Literature DB >> 5277091

Dissociation of mammalian polyribosomes into subunits by puromycin.

Abstract

Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0 degrees C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl tRNA, but were unable to continue the translation of mRNA. The polysomes were then heated to 37 degrees C, when they dissociated completely into subunits. Similar treatment without puromycin resulted in only partial dissociation.

Entities: Chemical Species

Mesh：

Substances：
Amino Acids

Year: 1971 PMID： 5277091 PMCID： PMC388945 DOI： 10.1073/pnas.68.2.390

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

1. Ribosome-catalyzed peptidyl transfer. Effects of cations and pH value.

Authors: B E Maden; R E Monro
Journal: Eur J Biochem Date: 1968-11

2. Release of transfer RNA during peptide chain elongation.

Authors: J Lucas-Lenard; A L Haenni
Journal: Proc Natl Acad Sci U S A Date: 1969-05 Impact factor: 11.205

3. The effect of incubation with puromycin on the dissociation of rat liver ribosomes into active subunits.

Authors: G R Lawford
Journal: Biochem Biophys Res Commun Date: 1969-09-24 Impact factor: 3.575

4. Distribution of radioactivity between the acid-soluble pool and the pools of RNA in the nuclear, nonsedimethable and ribosome fractions of rat liver after a single injection of lebaled orotic acid.

Authors: G Blobel; V R Potter
Journal: Biochim Biophys Acta Date: 1968-08-23

5. Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.

Authors: A K Falvey; T Staehelin
Journal: J Mol Biol Date: 1970-10-14 Impact factor: 5.469

6. Active hybrid 80 s particles formed from subunits of rat, rabbit and protozoan (Tetrahymena pyriformis) ribosomes.

Authors: T E Martin; I G Wool
Journal: J Mol Biol Date: 1969-07-14 Impact factor: 5.469

7. Purification and properties of dog pancreas ribosomes and subunits.

Authors: S R Dickman; E Bruenger
Journal: Biochemistry Date: 1969-08 Impact factor: 3.162

8. Factors influencing the puromycin-induced release of protein from liver ribosomes.

Authors: T Hultin
Journal: Biochim Biophys Acta Date: 1966-09

9. Studies on free and membrane-bound ribosomes in rat liver. II. Interaction of ribosomes and membranes.

Authors: G Blobel; V R Potter
Journal: J Mol Biol Date: 1967-06-14 Impact factor: 5.469

10. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. I. Location of the polypeptides within ribosomes.

Authors: G Blobel; D D Sabatini
Journal: J Cell Biol Date: 1970-04 Impact factor: 10.539

182 in total

1. Structural elements in the internal ribosome entry site of Plautia stali intestine virus responsible for binding with ribosomes.

Authors: Takashi Nishiyama; Hiroshi Yamamoto; Norihiro Shibuya; Yoshinori Hatakeyama; Akira Hachimori; Toshio Uchiumi; Nobuhiko Nakashima
Journal: Nucleic Acids Res Date: 2003-05-01 Impact factor: 16.971

2. Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP.

Authors: Shinya Kurata; Klaus H Nielsen; Sarah F Mitchell; Jon R Lorsch; Akira Kaji; Hideko Kaji
Journal: Proc Natl Acad Sci U S A Date: 2010-06-01 Impact factor: 11.205

3. Ribosomal proteins mediate the hepatitis C virus IRES-HeLa 40S interaction.

Authors: Geoff A Otto; Peter J Lukavsky; Alissa M Lancaster; Peter Sarnow; Joseph D Puglisi
Journal: RNA Date: 2002-07 Impact factor: 4.942

4. In vivo association of the stability control protein alphaCP with actively translating mRNAs.

Authors: Xinjun Ji; Jian Kong; Stephen A Liebhaber
Journal: Mol Cell Biol Date: 2003-02 Impact factor: 4.272

5. Biochemical and functional analysis of a 9-nt RNA sequence that affects translation efficiency in eukaryotic cells.

Authors: Stephen A Chappell; Gerald M Edelman; Vincent P Mauro
Journal: Proc Natl Acad Sci U S A Date: 2004-06-21 Impact factor: 11.205

6. Primary role for endoplasmic reticulum-bound ribosomes in cellular translation identified by ribosome profiling.

Authors: David W Reid; Christopher V Nicchitta
Journal: J Biol Chem Date: 2011-12-23 Impact factor: 5.157

7. Nonribosomal proteins associated with eukaryotic native small ribosomal subunits.

Authors: C Freienstein; G Blobel
Journal: Proc Natl Acad Sci U S A Date: 1975-09 Impact factor: 11.205

8. Requirement for extraction of polyribosomes from barley tissue.

Authors: M D Breen; E I Whitehead; D G Kenefick
Journal: Plant Physiol Date: 1972-05 Impact factor: 8.340

9. Binding of rat liver and hepatoma polyribosomes to stripped rough endoplasmic reticulum in vitro. Biological or an artifact?

Authors: A A Hochberg; F W Stratman; R N Zahlten; H P Morris; H A Lardy
Journal: Biochem J Date: 1972-11 Impact factor: 3.857

10. Protein initiation in eukaryotes: formation and function of a ternary complex composed of a partially purified ribosomal factor, methionyl transfer RNA, and guanosine triphosphate.

Authors: D H Levin; D Kyner; G Acs
Journal: Proc Natl Acad Sci U S A Date: 1973-01 Impact factor: 11.205