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Literature DB >> 4897025

Release of transfer RNA during peptide chain elongation.

Abstract

In amino acid polymerization, the tRNA donating its peptidyl moiety to the neighboring aminoacyl-tRNA must be released from the ribosome for further growth of the polypeptide chain. It was not known at what stage of peptide elongation this tRNA is released. To study this question, we prepared ac-C(14)-Phe-H(3)-tRNA using H(3)-tRNA isolated from E. coli strain 15THU grown in the presence of H(3)-uracil, and followed the fate of the H(3)-tRNA during chain growth. Our results indicate that donor tRNA is released during the translocation step, mediated by the soluble factor G and GTP.

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Year: 1969 PMID： 4897025 PMCID： PMC534040 DOI： 10.1073/pnas.63.1.93

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. A simple method for isolating highly purified yeast phenylalanine transfer ribonucleic acid.

Authors: E Wimmer; I H Maxwell; G M Tener
Journal: Biochemistry Date: 1968-07 Impact factor: 3.162

2. The interrelationship between guanosine triphosphatase and amino acid polymerization.

Authors: Y Nishizuka; F Lipmann
Journal: Arch Biochem Biophys Date: 1966-09-26 Impact factor: 4.013

3. Comparison of guanosine triphosphate split and polypeptide synthesis with a purified E. coli system.

Authors: Y Nishizuka; F Lipmann
Journal: Proc Natl Acad Sci U S A Date: 1966-01 Impact factor: 11.205

4. Separation of three microbial amino acid polymerization factors.

Authors: J Lucas-Lenard; F Lipmann
Journal: Proc Natl Acad Sci U S A Date: 1966-06 Impact factor: 11.205

5. Requirement of granosine 5'-triphosphate for ribosomal binding of aminoacyl-SRNA.

Authors: J Lucas-Lenard; A L Haenni
Journal: Proc Natl Acad Sci U S A Date: 1968-02 Impact factor: 11.205

6. Initiation and protein synthesis: translation of di- and tri-codon messengers.

Authors: R W Erbe; P Leder
Journal: Biochem Biophys Res Commun Date: 1968-06-10 Impact factor: 3.575

7. Reversal by GTP of soluble RNA inhibition of polyphenylalanine synthesis.

Authors: N W Seeds; T W Conway
Journal: Biochem Biophys Res Commun Date: 1966-04-19 Impact factor: 3.575

8. A competitive inhibitor of the GTP reaction in protein synthesis.

Authors: J W Hershey; R E Monro
Journal: J Mol Biol Date: 1966-06 Impact factor: 5.469

9. Initiation of protein synthesis II. A convenient assay for the ribosome-dependent synthesis of N-formyl-C14-methionylpuromycin.

Authors: P Leder; H Bursztyn
Journal: Biochem Biophys Res Commun Date: 1966-10-20 Impact factor: 3.575

10. The behaviour of acetylphenylalanyl soluble ribonucleic acid in polyphenylalanine synthesis.

Authors: A L Haenni; F Chapeville
Journal: Biochim Biophys Acta Date: 1966-01-18

16 in total

1. Bacterial ribosome.

Authors: M Nomura
Journal: Bacteriol Rev Date: 1970-09

2. Release of tRNA from ribosomes by a factor other than G factor.

Authors: H Ishitsuka; A Kaji
Journal: Proc Natl Acad Sci U S A Date: 1970-05 Impact factor: 11.205

3. Conformational changes in ribosomes during protein synthesis.

Authors: M H Schreier; H Noll
Journal: Proc Natl Acad Sci U S A Date: 1971-04 Impact factor: 11.205

4. Translocation of messenger RNA and "accommodation" of fMet-tRNA.

Authors: S S Thach; R E Thach
Journal: Proc Natl Acad Sci U S A Date: 1971-08 Impact factor: 11.205

5. Dissociation of mammalian polyribosomes into subunits by puromycin.

Authors: G Blobel; D Sabatini
Journal: Proc Natl Acad Sci U S A Date: 1971-02 Impact factor: 11.205

6. New aspects of the ribosomal elongation cycle.

Authors: K H Nierhaus
Journal: Mol Cell Biochem Date: 1984 Impact factor: 3.396

7. EF-G-catalyzed translocation of anticodon stem-loop analogs of transfer RNA in the ribosome.

Authors: S Joseph; H F Noller
Journal: EMBO J Date: 1998-06-15 Impact factor: 11.598

8. Synthesis and aminoacylation of 3'-amino-3'-deoxy transfer RNA and its activity in ribosomal protein synthesis.

Authors: T H Fraser; A Rich
Journal: Proc Natl Acad Sci U S A Date: 1973-09 Impact factor: 11.205

9. Chemical probing of the tRNA--ribosome complex.

Authors: D A Peattie; W Herr
Journal: Proc Natl Acad Sci U S A Date: 1981-04 Impact factor: 11.205

10. The interaction of elongation factor G with N-acetylphenylalanyl transfer RNA-ribosome complexes.

Authors: J Modolell; B Cabrer; D Váquez
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205