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Literature DB >> 5269241

Temporal resolution of individual steps in an enzymic reaction at low temperature.

Abstract

Media are described which make it possible to study mechanisms of enzyme action at low temperature. The applicability of techniques developed are illustrated by results obtained on formation kinetics of complexes produced by the interaction of horseradish peroxidase with hydrogen peroxide. It is shown that the various steps in the time course of the reaction can be readily resolved with isolation of each intermediate in concentrations sufficient to permit rate studies between consecutive steps. The potential of the method for elaboration of enzyme mechanisms is discussed.

Entities: Chemical Species

Mesh：

Substances：

Year: 1970 PMID： 5269241 PMCID： PMC283119 DOI： 10.1073/pnas.66.3.787

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

9 in total

1. ENZYMATIC REACTIONS BELOW 0-DEGREES OF ALPHA-CHYMOTRYPSIN IN METHANOL-WATER SOLVENTS.

Authors: B H BIELSKI; S FREED
Journal: Biochim Biophys Acta Date: 1964-08-26

2. OPTICALLY ACTIVE METALLOPROTEIN CHROMOPHORES. III. HEME AND NONHEME IRON PROTEINS.

Authors: D D ULMER; B L VALLEE
Journal: Biochemistry Date: 1963 Nov-Dec Impact factor: 3.162

3. The spectra of the enzyme-substrate complexes of catalase and peroxidase.

Authors: B CHANCE
Journal: Arch Biochem Biophys Date: 1952-12 Impact factor: 4.013

4. [Low-temperature spectroscopic study of D-aminoacid oxidase].

Authors: T Shiga; M Layani; P Douzou
Journal: Bull Soc Chim Biol (Paris) Date: 1967

5. Optical rotatory dispersion of polyglutamic and polyuridylic acids at low temperatures in fluid hydro-alcoholic solvents.

Authors: F Travers; M P Debey; P Douzou; A M Michelson
Journal: Biochim Biophys Acta Date: 1969-11-11

6. Kinetics of association of carbon monoxide with haemoglobin at low temperature.

Authors: R Banerjee; P Douzou; A Lombard
Journal: Nature Date: 1968-01-06 Impact factor: 49.962

7. The effect of ligand binding and acid splitting on the optical rotatory dispersion of ferric horseradish peroxidase.

Authors: W D Ellis; H B Dunford
Journal: Can J Biochem Date: 1968-10

8. Synthetic polynucleotides in fluid supercooled aqueous alcohol solutions.

Authors: P Douzou; G H Hoa; A M Michelson
Journal: Biochim Biophys Acta Date: 1969-06-17

9. Chemical-biochemical signal and noise.

Authors: S Freed
Journal: Science Date: 1965-10-29 Impact factor: 47.728

9 in total

1. Cryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity.

Authors: P Douzou; C Balny
Journal: Proc Natl Acad Sci U S A Date: 1977-06 Impact factor: 11.205

2. Resolution and Characterization of Chemical Steps in Enzyme Catalytic Sequences by Using Low-Temperature and Time-Resolved, Full-Spectrum EPR Spectroscopy in Fluid Cryosolvent and Frozen Solution Systems.

Authors: Miao Wang; Chen Zhu; Meghan Kohne; Kurt Warncke
Journal: Methods Enzymol Date: 2015-09-14 Impact factor: 1.600

Review 8. Enzymology at sub-zero temperatures.

Authors: P Douzou
Journal: Mol Cell Biochem Date: 1973-05-11 Impact factor: 3.396

9. Testing cosolvent cryoenzymology on multi-enzyme systems.

Authors: P Debey; I C Gunsalus; P Douzou
Journal: Mol Cell Biochem Date: 1979-07-15 Impact factor: 3.396

9 in total

Temporal resolution of individual steps in an enzymic reaction at low temperature.

1. ENZYMATIC REACTIONS BELOW 0-DEGREES OF ALPHA-CHYMOTRYPSIN IN METHANOL-WATER SOLVENTS.

2. OPTICALLY ACTIVE METALLOPROTEIN CHROMOPHORES. III. HEME AND NONHEME IRON PROTEINS.

3. The spectra of the enzyme-substrate complexes of catalase and peroxidase.

4. [Low-temperature spectroscopic study of D-aminoacid oxidase].

5. Optical rotatory dispersion of polyglutamic and polyuridylic acids at low temperatures in fluid hydro-alcoholic solvents.

6. Kinetics of association of carbon monoxide with haemoglobin at low temperature.

7. The effect of ligand binding and acid splitting on the optical rotatory dispersion of ferric horseradish peroxidase.

8. Synthetic polynucleotides in fluid supercooled aqueous alcohol solutions.

9. Chemical-biochemical signal and noise.

1. Cryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity.

2. Resolution and Characterization of Chemical Steps in Enzyme Catalytic Sequences by Using Low-Temperature and Time-Resolved, Full-Spectrum EPR Spectroscopy in Fluid Cryosolvent and Frozen Solution Systems.

3. Functional intermediates in reaction of cytochrome oxidase with oxygen.

4. Low temperature kinetics of H+ changes of bacterial rhodopsin.

5. pH-jump studies at subzero temperatures on an intermediate in the reaction of xanthine oxidase with xanthine.

6. Thermodynamics of the two step formation of horseradish peroxidase compound I.

7. Enzyme assay in microsomes below zero degrees.

Review 8. Enzymology at sub-zero temperatures.

9. Testing cosolvent cryoenzymology on multi-enzyme systems.