Literature DB >> 18734

Cryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity.

P Douzou, C Balny.   

Abstract

Water-soluble polyelectrolytes in interaction with proteins are described. These polyelectrolytes make it possible to investigate enzyme-catalyzed reactions in cooled mixed solvents without the usual effects of their organic solvent component on enzyme specific activity. The applicability of techniques developed is illustrated by results obtained on several systems. The possibility of an electrostatic "sorting out" of solvents and its potentialities in cryoenzymology are discussed.

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Year:  1977        PMID： 18734      PMCID： PMC432157          DOI： 10.1073/pnas.74.6.2297

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  [Ionic control of biochemical reactions].

Authors:  P Douzou; P Maurel
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1976-06-21

2.  Catalytic implications of electrostatic potentials: the lytic activity of lysozymes as a model.

Authors:  P Maurel; P Douzou
Journal:  J Mol Biol       Date:  1976-04-05       Impact factor: 5.469

3.  THE ANTI-TRYPTIC PROPERTIES OF HEPARIN.

Authors:  M K Horwitt
Journal:  Science       Date:  1940-07-26       Impact factor: 47.728

4.  Crystal structure of elastase-substrate complex at -- 55 degrees C.

Authors:  T Alber; G A Petsko; D Tsernoglou
Journal:  Nature       Date:  1976-09-23       Impact factor: 49.962

5.  Protein crystallography at sub-zero temperatures: lysozyme-substrate complexes in cooled mixed solvents.

Authors:  P Douzou; G H Hoa; G A Petsko
Journal:  J Mol Biol       Date:  1975-08-15       Impact factor: 5.469

6.  Protein crystallography at sub-zero temperatures: cryo-protective mother liquors for protein crystals.

Authors:  G A Petsko
Journal:  J Mol Biol       Date:  1975-08-15       Impact factor: 5.469

Review 7.  The use of subzero temperatures in biochemistry: slow reactions.

Authors:  P Douzou
Journal:  Methods Biochem Anal       Date:  1974

Review 8.  Protein-protein interaction and enzymatic activity.

Authors:  C Frieden
Journal:  Annu Rev Biochem       Date:  1971       Impact factor: 23.643

Review 9.  Interactions of polynucleotides and other polyelectrolytes with enzymes and other proteins.

Authors:  A D Elbein
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1974

10.  Enzyme assay in microsomes below zero degrees.

Authors:  P Debey; C Balny; P Douzou
Journal:  Proc Natl Acad Sci U S A       Date:  1973-09       Impact factor: 11.205
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  3 in total

1.  The effects of surface and macromolecular interactions on the kinetics of inactivation of trypsin and alpha-chymotrypsin.

Authors:  P Johnson; T L Whateley
Journal:  Biochem J       Date:  1981-01-01       Impact factor: 3.857

2.  Mechanistic studies of the yeast polyamine oxidase Fms1: kinetic mechanism, substrate specificity, and pH dependence.

Authors:  Mariya S Adachi; Jason M Torres; Paul F Fitzpatrick
Journal:  Biochemistry       Date:  2010-11-16       Impact factor: 3.162

3.  Cryoradiolysis and cryospectroscopy for studies of heme-oxygen intermediates in cytochromes p450.

Authors:  I G Denisov; Y V Grinkova; S G Sligar
Journal:  Methods Mol Biol       Date:  2012
  3 in total

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