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Literature DB >> 4147450

Enzyme assay in microsomes below zero degrees.

Abstract

Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0 degrees were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0 degrees .

Entities: Chemical Species

Mesh：

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Year: 1973 PMID： 4147450 PMCID： PMC427071 DOI： 10.1073/pnas.70.9.2633

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

9 in total

1. STUDIES ON THE MECHANISM OF MICROSOMAL TRIPHOSPHOPYRIDINE NUCLEOTIDE-CYTOCHROME C REDUCTASE.

Authors: B S MASTERS; H KAMIN; Q H GIBSON; C H WILLIAMS
Journal: J Biol Chem Date: 1965-02 Impact factor: 5.157

2. The temperature dependence of the enolase reaction with different activating metal ions.

Authors: B G MALMSTROM
Journal: Biochim Biophys Acta Date: 1955-10

3. Kinetics of cytochrome b5 in rat liver microsomes.

Authors: B CHANCE; G R WILLIAMS
Journal: J Biol Chem Date: 1954-08 Impact factor: 5.157

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Studies on the electron transport chain at subzero temperatures: electron transport at site 3.

Authors: M Erecińska; B Chance
Journal: Arch Biochem Biophys Date: 1972-07 Impact factor: 4.013

6. Turnover of constituents of the endoplasmic reticulum membranes of rat hepatocytes.

Authors: T Omura; P Siekevitz; G E Palade
Journal: J Biol Chem Date: 1967-05-25 Impact factor: 5.157

7. The influence of deuterium oxide and organic solvents on the interaction of respiratory chain components.

Authors: D D Tyler; R W Estabrook
Journal: J Biol Chem Date: 1966-04-25 Impact factor: 5.157

8. Temporal resolution of individual steps in an enzymic reaction at low temperature.

Authors: P Douzou; R Sireix; F Travers
Journal: Proc Natl Acad Sci U S A Date: 1970-07 Impact factor: 11.205

9. Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane.

Authors: J M Steim; M E Tourtellotte; J C Reinert; R N McElhaney; R L Rader
Journal: Proc Natl Acad Sci U S A Date: 1969-05 Impact factor: 11.205

9 in total

7 in total

Enzyme assay in microsomes below zero degrees.

1. STUDIES ON THE MECHANISM OF MICROSOMAL TRIPHOSPHOPYRIDINE NUCLEOTIDE-CYTOCHROME C REDUCTASE.

2. The temperature dependence of the enolase reaction with different activating metal ions.

3. Kinetics of cytochrome b5 in rat liver microsomes.

4. Protein measurement with the Folin phenol reagent.

5. Studies on the electron transport chain at subzero temperatures: electron transport at site 3.

6. Turnover of constituents of the endoplasmic reticulum membranes of rat hepatocytes.

7. The influence of deuterium oxide and organic solvents on the interaction of respiratory chain components.

8. Temporal resolution of individual steps in an enzymic reaction at low temperature.

9. Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane.

1. Cryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity.

2. Room temperature phosphorescence and the dynamic aspects of protein structure.

3. Testing cosolvent cryoenzymology on multi-enzyme systems.

4. Chemical mechanisms for cytochrome P-450 hydroxylation: evidence for acylation of heme-bound dioxygen.

5. Effect of temperature on histidine ammonia-lyase from a psychrophile, Pseudomonas putida.

6. The effects of methanol on the glutamate dehydrogenase reaction at 0 degrees C.

7. Cryoradiolysis and cryospectroscopy for studies of heme-oxygen intermediates in cytochromes p450.