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Literature DB >> 5263745

Changes in the physical state of DNA during replication in regenerating liver of the rat.

Abstract

Evidence is presented which demonstrates that DNA synthesis in regenerating liver is discontinuous. In pulse-labeling experiments from 30 seconds to 10 minutes, the earliest detectable intermediate in DNA replication appears to be double-stranded DNA which has a significant degree of single-stranded character probably due to the existence of gaps in the newly synthesized strand. The label begins to move into completely double-stranded DNA 10-30 minutes after the commencement of labeling. The average molecular weight of the single-stranded "unit" of DNA replication is determined to be approximately 7.5 million. Smaller units may be produced but not observed as a result of imperfect synchronization of DNA synthesis.

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Year: 1970 PMID： 5263745 PMCID： PMC286204 DOI： 10.1073/pnas.65.1.152

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

11 in total

1. Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.

Authors: R Okazaki; T Okazaki; K Sakabe; K Sugimoto; A Sugino
Journal: Proc Natl Acad Sci U S A Date: 1968-02 Impact factor: 11.205

2. Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA.

Authors: M Oishi
Journal: Proc Natl Acad Sci U S A Date: 1968-05 Impact factor: 11.205

6. Absence of single-stranded regions in deoxyribonucleic acid during the action of an adenosine triphosphate dependent deoxyribonuclease.

Authors: F G Winder; P A Sastry
Journal: Biochem J Date: 1971-12 Impact factor: 3.857

7. Selective precipitation of ribonucleic acid from a mixture of total cellular nucleic acids extracted from cultured mammalian cells.

Authors: P R Harrison
Journal: Biochem J Date: 1971-01 Impact factor: 3.857

7 in total

Changes in the physical state of DNA during replication in regenerating liver of the rat.

1. Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.

2. Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA.

3. Polydeoxynucleotide intermediates in DNA replication in regenerating liver.

4. Artefact in the measurement of the molecular weight of pulse labelled DNA.

5. Early events in the replication and integration of DNA into mammalian chromosomes.

6. Studies on the nature of replicating DNA of HeLa cells.

7. Chromatography of nucleic acids on hydroxyapatite.

8. DNA replication in vivo by a temperature-sensitive polynucleotide ligase mutant of T4.

9. Tables for estimating sedimentation through linear concentration gradients of sucrose solution.

10. Isolation of deoxyribonucleic acid from mammalian tissues.

1. Replication of DNA in mammalian chromosomes. III. Size and RNA content of Okazaki fragments.

2. Accumulation of an intermediate in DNA synthesis by HEp.2 cells treated with methyl methanesulfonate.

3. Sites of replication of chromosomal DNA in a eukaryotic cell.

4. Synthesis of deoxyribonucleic acid in BHK-21-C13 cells.

5. Repair of radiation-induced DNA strand scissions in Chlorella during the cell cycle.

6. Absence of single-stranded regions in deoxyribonucleic acid during the action of an adenosine triphosphate dependent deoxyribonuclease.

7. Selective precipitation of ribonucleic acid from a mixture of total cellular nucleic acids extracted from cultured mammalian cells.