Literature DB >> 4291998

Isolation of deoxyribonucleic acid from mammalian tissues.

K S Kirby, E A Cook.   

Abstract

1. DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol-cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides remained in solution when DNA was precipitated with 2-butoxyethanol in the presence of 0.5m-sodium chloride and 1.5m-sodium benzoate. 4. Ribosomal RNA was removed by precipitation in the presence of 3m-sodium chloride at 0 degrees , when DNA remained soluble.

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Year:  1967        PMID： 4291998      PMCID： PMC1270569          DOI： 10.1042/bj1040254

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  Preparation of some deoxyribonucleic acid-protein complexes from rat-liver homogenates.

Authors:  K S KIRBY
Journal:  Biochem J       Date:  1958-10       Impact factor: 3.857

2.  The preparation of deoxyribonucleic acids by the p-aminosalicylate-phenol method.

Authors:  K S KIRBY
Journal:  Biochim Biophys Acta       Date:  1959-11

3.  A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein.

Authors:  K S KIRBY
Journal:  Biochem J       Date:  1957-07       Impact factor: 3.857

4.  Sedimentation characteristics of rapidly labelled RNA from HeLa cells.

Authors:  K SCHERRER; J E DARNELL
Journal:  Biochem Biophys Res Commun       Date:  1962-06-04       Impact factor: 3.575

5.  Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver.

Authors:  J H Parish; K S Kirby
Journal:  Biochim Biophys Acta       Date:  1966-12-21

6.  Nucleoproteins. 2. Fractionation of deoxyribonucleoproteins into acidic proteins and lysine-rich histones associated with deoxyribonucleic acid.

Authors:  P M Frearson; K S Kirby
Journal:  Biochem J       Date:  1964-03       Impact factor: 3.857

7.  Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria.

Authors:  K S Kirby; E Fox-Carter; M Guest
Journal:  Biochem J       Date:  1967-07       Impact factor: 3.857

8.  The nucleic acids of Drosophila melanogaster.

Authors:  J R Hastings; K S Kirby
Journal:  Biochem J       Date:  1966-08       Impact factor: 3.857

9.  Induction of tumours by tannin extracts.

Authors:  K S KIRBY
Journal:  Br J Cancer       Date:  1960-03       Impact factor: 7.640

10.  STUDIES ON NUCLEIC ACIDS DURING CARCINOGENESIS.

Authors:  M A O'SULLIVAN; K S KIRBY
Journal:  Br J Cancer       Date:  1964-12       Impact factor: 7.640
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  44 in total

1.  Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.

Authors:  R W Pero; T Bryngelsson; C Bryngelsson; A Deutsch; A Nordén; A Norgren
Journal:  Nucleic Acids Res       Date:  1975-07       Impact factor: 16.971

2.  Intracellular distribution and sedimentation properties of virus-specific RNA in two clones of BHK cells transformed by polyoma virus.

Authors:  I H Maxwell
Journal:  J Virol       Date:  1976-05       Impact factor: 5.103

3.  Artifacts in the centrifugation of ribosomal and heterogenous ribonucleic acid in "99%-dimethyl sulphoxide" gradients.

Authors:  I H Maxwell
Journal:  Biochem J       Date:  1976-02-01       Impact factor: 3.857

4.  Comparison of 2,2-bis(bromomethyl)-1,3-propanediol induced genotoxicity in UROtsa cells and primary rat hepatocytes: relevance of metabolism and oxidative stress.

Authors:  Weixi Kong; Pengfei Gu; Gabriel A Knudsen; I Glenn Sipes
Journal:  Toxicol Lett       Date:  2013-08-13       Impact factor: 4.372

5.  Removal of RNase activity from DNase by affinity chromatography on agarose coupled aminophenylphosphoryl-uridine-2' (3')-phosphate.

Authors:  I H Maxwell; F Maxwell; W E Hahn
Journal:  Nucleic Acids Res       Date:  1977-01       Impact factor: 16.971

6.  Stability of the Apoproteins of Light-Harvesting Complex I and II during Biogenesis of Thylakoids in the Chlorophyll b-less Barley Mutant Chlorina f2.

Authors:  S. Preiss; J. P. Thornber
Journal:  Plant Physiol       Date:  1995-03       Impact factor: 8.340

7.  Caffeine inhibits excision of 7-bromomethylbenz (a) anthracene-DNA adducts from exponentially growing but not from stationary phase Chinese hamster cells.

Authors:  F Friedlos; J J Roberts
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

8.  Population heterogeneity of the Hpa I restriction site associated with the beta globin gene: implications for prenatal diagnosis.

Authors:  S R Panny; A F Scott; K D Smith; J A Phillips; H H Kazazian; C C Talbot; C D Boehm
Journal:  Am J Hum Genet       Date:  1981-01       Impact factor: 11.025

9.  Altered gene expression in three plant species in response to treatment with Nep1, a fungal protein that causes necrosis.

Authors:  Sarah E Keates; Todd A Kostman; James D Anderson; Bryan A Bailey
Journal:  Plant Physiol       Date:  2003-07       Impact factor: 8.340

10.  Benzyladenine modulation of the expression of two genes for nuclear-encoded chloroplast proteins in Lemna gibba: Apparent post-transcriptional regulation.

Authors:  S Flores; E M Tobin
Journal:  Planta       Date:  1986-09       Impact factor: 4.116
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