Literature DB >> 5256220

A specific anti-proinsulin serum and the presence of proinsulin in calf serum.

C C Yip, J Logothetopoulos.   

Abstract

A specific antibody against proinsulin has been obtained by adsorbing the original anti-proinsulin guinea pig serum with a solid immunosorbent of Sephadex-insulin. The specificity of the antibody against antigenic determinants of proinsulin was established by radioimmunoassay and by passive cutaneous anaphylaxis (PCA) tests. The presence of proinsulin in calf serum has been demonstrated by (a) gel filtration of an acid alcohol extract of the serum followed by immunoassays of the fractions using anti-insulin or the specific anti-proinsulin serum, and (b) direct assay of calf serum with the specific anti-proinsulin serum.

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Year:  1969        PMID： 5256220      PMCID： PMC277814          DOI： 10.1073/pnas.62.2.415

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

2.  The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma.

Authors:  D F Steiner; P E Oyer
Journal:  Proc Natl Acad Sci U S A       Date:  1967-02       Impact factor: 11.205

3.  "Big insulin": a new component of plasma insulin detected by immunoassay.

Authors:  J Roth; P Gorden; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1968-09       Impact factor: 11.205

4.  Evidence for proinsulin in human urine and serum.

Authors:  A H Rubenstein; S Cho; D F Steiner
Journal:  Lancet       Date:  1968-06-22       Impact factor: 79.321

5.  Terminal amino group determination, peptide mapping, biological and immunological activity of an insulin-like protein and the amino acid compositions of its two S-sulfo-polypeptide chains.

Authors:  C C Yip
Journal:  Arch Biochem Biophys       Date:  1968-09-20       Impact factor: 4.013

6.  Amino acid composition of bovine 'proinsulin'.

Authors:  C C Yip; B J Lin
Journal:  Biochem Biophys Res Commun       Date:  1967-11-17       Impact factor: 3.575

7.  Chemical coupling of enzymes to cross-linked dextran ('Sephadex').

Authors:  R Axén; J Porath
Journal:  Nature       Date:  1966-04-23       Impact factor: 49.962

8.  Porcine proinsulin: characterization and amino acid sequence.

Authors:  R E Chance; R M Ellis; W W Bromer
Journal:  Science       Date:  1968-07-12       Impact factor: 47.728

9.  Insulin biosynthesis: evidence for a precursor.

Authors:  D F Steiner; D Cunningham; L Spigelman; B Aten
Journal:  Science       Date:  1967-08-11       Impact factor: 47.728
  9 in total
  6 in total

1.  Assay of insulin-like activity by the isolated fat cell method. IV. The biological activity of proinsulin.

Authors:  J Gliemann; H H Sorensen
Journal:  Diabetologia       Date:  1970-10       Impact factor: 10.122

2.  Characterization of circulating insulin and proinsulin-binding antibodies in autoimmune hypoglycemia.

Authors:  J Goldman; D Baldwin; A H Rubenstein; D D Klink; W G Blackard; L K Fisher; T F Roe; J J Schnure
Journal:  J Clin Invest       Date:  1979-05       Impact factor: 14.808

3.  Circulating insulin: th proinsulin-like properties of "big" insulin in patients withou islet cell tumors.

Authors:  B M Sherman; P Gorden; J Roth; P Freychet
Journal:  J Clin Invest       Date:  1971-04       Impact factor: 14.808

4.  The metabolism of proinsulin and insulin by the liver.

Authors:  A H Rubenstein; L A Pottenger; M Mako; G S Getz; D F Steiner
Journal:  J Clin Invest       Date:  1972-04       Impact factor: 14.808

5.  A bovine pancreatic enzyme catalyzing the conversion of proinsulin to insulin.

Authors:  C C Yip
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205

6.  Biosynthesis of insulin in bovine fetal pancreatic slices: the incorporation of tritiated leucine into a single-chain proinsulin, a double-chain intermediate, and insulin in subcellular fractions.

Authors:  A K Tung; C C Yip
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205
  6 in total

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