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Literature DB >> 5657063

Porcine proinsulin: characterization and amino acid sequence.

Abstract

Proinsulin in nearly homogeneous form has been isolated from a preparation of porcine insulin. A molecular weight close to 9100 was calculated from the amino acid composition and from sedimentation-equilibrium studies. Through the action of trypsin this single-chain protein is transformed to desalanine insulin by cleavage of a polypeptide chain connecting the carboxy-terminus of the B chain to the amino-terminus of the A chain of insulin. The amino acid sequence of this connecting peptide was found to be Arg-Arg-Glu-Ala-Gln-Asn-Pro-Gln-Ala-Gly-Ala-Val-Glu-Leu-Gly-Gly-Gly-Leu-Gly-Gly-Leu-Gln-Ala-Leu-Ala-Leu-Glu-Gly-Pro-Pro-Gln-Lys-Arg.

Entities: Chemical Disease Gene

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Year: 1968 PMID： 5657063 DOI： 10.1126/science.161.3837.165

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

58 in total

1. Editorial: Lessons From the Classic Scientific Literature.

Authors: Raghavendra G Mirmira
Journal: Mol Endocrinol Date: 2015-10

2. Structure and receptor-binding activity of insulin from a holostean fish, the bowfin (Amia calva).

Authors: J M Conlon; J H Youson; J Whittaker
Journal: Biochem J Date: 1991-05-15 Impact factor: 3.857

3. Kex2-like endoproteases PC2 and PC3 accurately cleave a model prohormone in mammalian cells: evidence for a common core of neuroendocrine processing enzymes.

Authors: L Thomas; R Leduc; B A Thorne; S P Smeekens; D F Steiner; G Thomas
Journal: Proc Natl Acad Sci U S A Date: 1991-06-15 Impact factor: 11.205

Porcine proinsulin: characterization and amino acid sequence.

1. Editorial: Lessons From the Classic Scientific Literature.

2. Structure and receptor-binding activity of insulin from a holostean fish, the bowfin (Amia calva).

3. Kex2-like endoproteases PC2 and PC3 accurately cleave a model prohormone in mammalian cells: evidence for a common core of neuroendocrine processing enzymes.

4. Morphine-like substances and the pituitary gland.

5. Growth hormone and metasomatotrophic diabetes: effects on insulin and proinsulin of serum and pancreas in dogs.

6. Human serum proinsulin.

7. In vitro conversion of proinsulin to insulin by cathepsin B and role of C-peptide.

8. Trypsin-catalysed formation of pig des-(23-63)-proinsulin from desoctapeptide-(B23-30)-insulin.

Review 9. The evolution of proteinase substrates with special reference to dipeptidylpeptidase IV.

10. Characterization of proinsulin-insulin intermediates in human plasma.