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Literature DB >> 5085550

Biomethylation of deoxyribonucleic acid in cultured human tumour cells (HeLa). Methylated bases other than 5-methylcytosine not detected.

P D Lawley, A R Crathorn, S A Shah, B A Smith.

Abstract

1. Incorporation of methyl groups from [methyl-(14)C]methionine into DNA of dividing HeLa cells was investigated, essentially by the procedures of Culp, et al. (1970). 2. Contrary to the report of the latter, but in agreement with other work on biomethylation of mammalian DNA, 5-methylcytosine was the sole methylated base detected. 3. Chromatographic separations of 3-methylcytosine from 5-methylcytosine and purines are discussed.

Entities: Chemical Disease Species

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Year: 1972 PMID： 5085550 PMCID： PMC1173577 DOI： 10.1042/bj1280133

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

12 in total

Review 1. Carcinogenic nitroso compounds.

Authors: P N Magee; J M Barnes
Journal: Adv Cancer Res Date: 1967 Impact factor: 6.242

2. Rapid separation of nucleosides and nucleotides by cation-exchange column chromatography.

Authors: E Junowicz; J H Spencer
Journal: J Chromatogr Date: 1969-10-28

3. Ribonucleic acid polymerase reactions with methylated polycytidylic acid templates.

Authors: D B Ludlum; R C Wilhelm
Journal: J Biol Chem Date: 1968-05-25 Impact factor: 5.157

4. Methylated bases in DNA of animal origin.

Authors: L A Culp; E Dore; G M Brown
Journal: Arch Biochem Biophys Date: 1970-01 Impact factor: 4.013

Review 5. Effects of some chemical mutagens and carcinogens on nucleic acids.

Authors: P D Lawley
Journal: Prog Nucleic Acid Res Mol Biol Date: 1966

6. Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides.

Authors: A Loveless
Journal: Nature Date: 1969-07-12 Impact factor: 49.962

7. Methylation of nucleic acids in HeLa cells.

Authors: G M Brown; G Attardi
Journal: Biochem Biophys Res Commun Date: 1965-07-26 Impact factor: 3.575

8. Methylation of ribonucleic acid by the carcinogens dimethyl sulphate, N-methyl-N-nitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine. Comparisons of chemical analyses at the nucleoside and base levels.

Authors: P D Lawley; S A Shah
Journal: Biochem J Date: 1972-06 Impact factor: 3.857

9. Methylation of deoxyribonucleic acid in cultured mammalian cells by N-methyl-N'-nitro-N-nitrosoguanidine. The influence of cellular thiol concentrations on the extent of methylation and the 6-oxygen atom of guanine as a site of methylation.

Authors: P D Lawley; C J Thatcher
Journal: Biochem J Date: 1970-02 Impact factor: 3.857

Biomethylation of deoxyribonucleic acid in cultured human tumour cells (HeLa). Methylated bases other than 5-methylcytosine not detected.

Review 1. Carcinogenic nitroso compounds.

2. Rapid separation of nucleosides and nucleotides by cation-exchange column chromatography.

3. Ribonucleic acid polymerase reactions with methylated polycytidylic acid templates.

4. Methylated bases in DNA of animal origin.

Review 5. Effects of some chemical mutagens and carcinogens on nucleic acids.

6. Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides.

7. Methylation of nucleic acids in HeLa cells.

8. Methylation of ribonucleic acid by the carcinogens dimethyl sulphate, N-methyl-N-nitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine. Comparisons of chemical analyses at the nucleoside and base levels.

9. Methylation of deoxyribonucleic acid in cultured mammalian cells by N-methyl-N'-nitro-N-nitrosoguanidine. The influence of cellular thiol concentrations on the extent of methylation and the 6-oxygen atom of guanine as a site of methylation.

10. Isolation of deoxyribonucleic acid from mammalian tissues.

1. The purification and properties of adenosine 5'-sulphatophosphate sulphohydrolase from bovine liver.

2. DNA methylase from HeLa cell nuclei.

3. DNA methylation in adenovirus, adenovirus-transformed cells, and host cells.

Review 4. N6-methyladenine: the other methylated base of DNA.

5. New methods for detection of low levels of DNA damage in human populations.

6. Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.