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Literature DB >> 5002312

Staphylococcal enterotoxin B: solid-phase radioimmunoassay.

H M Johnson, J A Bukovic, P E Kauffman, J T Peeler.

Abstract

An immunoassay employing (125)I-labeled enterotoxin B and polystyrene tubes coated with specific antibody was used for assaying purified and crude enterotoxin. Antibody was adsorbed to untreated polystyrene tubes. Unlabeled enterotoxin competed with (125)I-labeled enterotoxin for antibody-combining sites. The uptake of (125)I-labeled toxin reflected the concentration of unlabeled toxin present. The test is sensitive to 1 to 5 ng of purified and crude enterotoxin B per ml, and cross-reactions with heterologous enterotoxins did not interfere with the specificity. This test possesses the combination of sensitivity and objectivity absent in current methods for assaying enterotoxin and provides a model for investigating other enterotoxin serotypes.

Entities: Chemical

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Year: 1971 PMID： 5002312 PMCID： PMC376429 DOI： 10.1128/am.22.5.837-841.1971

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

9 in total

18 in total

4. Comparative inhibitory effects of antigen and antibody in the staphylococcal enterotoxin solid-phase radioimmunoassay system.

Authors: J A Bukovic; H M Johnson; J T Peeler
Journal: Appl Microbiol Date: 1975-06

Staphylococcal enterotoxin B: solid-phase radioimmunoassay.

1. QUANTITATIVE DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B IN FOOD BY GEL-DIFFUSION METHODS.

2. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

3. DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD.

4. The use of a wate-soluble carbodiimide as a coupling reagent in the passive hemagglutination test.

5. Standardization of the chloramine-T method of protein iodination.

6. Solid phase radioimmunoassay of human beta 1C globulin.

7. Enterotoxin B: serological assay in cultures by passive hemagglutination.

8. Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods.

9. Solid-phase radioimmunoassay in antibody-coated tubes.

1. Double-antibody radioimmunoassay for staphylococcal enterotoxin C2.

2. Iodination of staphylococcal enterotoxin B by use of chloramine-T.

3. Stability of 125I-labeled staphylococcal enterotoxins in solid-phase radioimmunoassay.

4. Comparative inhibitory effects of antigen and antibody in the staphylococcal enterotoxin solid-phase radioimmunoassay system.

5. Modified radioimmunoassay determination for staphylococcal enterotoxin B in foods.

6. Staphylococcal enterotoxin C: solid-phase radioimmunoassay.

7. Detection of staphylococcal enterotoxin in gastric juice.

8. Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay.

9. Staphylococcal food poisoning and botulism.

10. Determination of staphylococcal enterotoxin A in cheddar cheese produced without starter activity.