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Literature DB >> 500121

Isolation of serum amyloid P-component (protein SAP) in the mouse.

Abstract

Serum amyloid P-component (protein SAP) was associated from mouse serum and ascitic fluids by calcium-dependent affinity chromatography on Sepharose 4B (Pharmacia) followed by gel filtration on Ultrogel AcA44 (LKB) in the presence of EDTA. It was homogenous on gradient polyacrylamide gel and sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis, and induced a monospecific antiserum in the rabbit. The related protein, C-reactive protein (CRP) was present in only trace amounts not exceeding 2 mug/ml of serum even during acute phase reactions. It was isolated by calcium-dependent affinity chromatography on insolubilized pneumococcal C-polysaccharide. The availability of these isolated mouse proteins and their respective antisera will facilitate investigation of their function.

Entities: Chemical Disease Gene Species

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Year: 1979 PMID： 500121 PMCID： PMC1457743

Source DB: PubMed Journal: Immunology ISSN： 0019-2805 Impact factor: 7.397

9 in total

1. Isolation of C-reactive protein by affinity chromatography.

Authors: M B Pepys; A C Dash; M J Ashley
Journal: Clin Exp Immunol Date: 1977-10 Impact factor: 4.330

2. Characterization of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying cyclic pentameric symmetry (pentraxins).

Authors: A P Osmand; B Friedenson; H Gewurz; R H Painter; T Hofmann; E Shelton
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

3. Analogues in other mammals and in fish of human plasma proteins, C-reactive protein and amyloid P component.

Authors: M B Pepys; A C Dash; T C Fletcher; N Richardson; E A Munn; A Feinstein
Journal: Nature Date: 1978-05-11 Impact factor: 49.962

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. A fluorescent immunoassay for the quantification of C-reactive protein.

Authors: R Siboo; E Kulisek
Journal: J Immunol Methods Date: 1978 Impact factor: 2.303

6. Comparative clinical study of protein SAP (amyloid P component) and C-reactive protein in serum.

Authors: M B Pepys; A C Dash; R E Markham; H C Thomas; B D Williams; A Petrie
Journal: Clin Exp Immunol Date: 1978-04 Impact factor: 4.330

7. Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.

Authors:
Journal: J Exp Med Date: 1975-11-01 Impact factor: 14.307

8. Isolation from individual A/J mice of anti-rho-azophenylarsonate antibodies bearing a cross-reactive idiotype.

Authors: A S Tung; A Nisonoff
Journal: J Exp Med Date: 1975-01-01 Impact factor: 14.307

9. IgM-producing tumors in the BALB-c mouse: a model for B-cell maturation.

Authors: J Andersson; J Buxbaum; R Citronbaum; S Douglas; L Forni; F Melchers; B Pernis; D Stott
Journal: J Exp Med Date: 1974-09-01 Impact factor: 14.307

9 in total

41 in total

1. Studies on type II collagen induced arthritis in mice.

Authors: W Paska; K J McDonald; M Croft
Journal: Agents Actions Date: 1986-06

2. Defective production of leucocytic endogenous mediator (interleukin 1) by peripheral blood leucocytes of patients with systemic sclerosis, systemic lupus erythematosus, rheumatoid arthritis and mixed connective tissue disease.

Authors: J T Whicher; A M Gilbert; C Westacott; C Hutton; P A Dieppe
Journal: Clin Exp Immunol Date: 1986-07 Impact factor: 4.330

3. SAP suppresses the development of experimental autoimmune encephalomyelitis in C57BL/6 mice.

Authors: Zhe Ji; Zun-Ji Ke; Jian-Guo Geng
Journal: Immunol Cell Biol Date: 2011-06-07 Impact factor: 5.126

4. Studies in vivo and in vitro of serum amyloid P component in normals and in a patient with AA amyloidosis.

Authors: P N Hawkins; G A Tennent; P Woo; M B Pepys
Journal: Clin Exp Immunol Date: 1991-05 Impact factor: 4.330

5. Effect of bacterial endotoxin on body temperature, plasma zinc and plasma concentrations of the acute-phase protein serum amyloid p component in mice.

Authors: S Poole; A H Gordon; M Baltz; B E Stenning
Journal: Br J Exp Pathol Date: 1984-08