Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Some features of the bacterial membrane studied with the aid of a new fractionation technique.

Literature DB >> 5000708

Some features of the bacterial membrane studied with the aid of a new fractionation technique.

Abstract

Membranes of Bacillus megaterium and other bacteria were bound to crystals of cadmium lauroylsarcosinate and were resolved into nine lipoprotein fractions by elution with potassium chloride and sodium deoxycholate solutions. The fractions differed widely in protein/lipid ratio. Some major protein species were probably common to all the fractions, but the phospholipid composition showed some variation. Electron-microscopic examination after negative staining revealed that material in certain fractions was in the form of particles of diameter about 12nm, and other fractions consisted of amorphous aggregates. The composition of the membrane in terms of the nine fractions was influenced markedly by the conditions of culture of the bacteria. Radioactive-labelling experiments suggested that some fractions served as precursors of others, and one fraction appeared to contain the attachment points of the DNA.

Entities: Chemical Species

Mesh：

Substances：

Year: 1971 PMID： 5000708 PMCID： PMC1176763 DOI： 10.1042/bj1220197

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

21 in total

1. Some enzymic activities and chemical properties of the mesosomes and cytoplasmic membranes of Bacillus licheniformis 6346.

Authors: D A Reaveley; H J Rogers
Journal: Biochem J Date: 1969-06 Impact factor: 3.857

Some features of the bacterial membrane studied with the aid of a new fractionation technique.

1. Some enzymic activities and chemical properties of the mesosomes and cytoplasmic membranes of Bacillus licheniformis 6346.

2. Properties of the growing point region in the bacterial chromosome.

3. Studies of the phospholipids and morphology of protoplasts of Bacillus megaterium.

4. Isolation and characterization of bacterial membranes.

5. Membrane attachment of the chromosome replication origin in Bacillus subtilis.

6. Shear sensitivity of the E. coli genome: multiple membrane attachment points of the E. coli DNA.

7. Evidence for an intermediate stage in the biosynthesis of the Salmonella O-antigen.

8. Lipid synthesis in relation to the cell cycle of Bacillus megaterium KM and Escherichia coli.

9. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance.

10. Phospholipid alterations during growth of Escherichia coli.

1. Isolation and properties of mesosomal membrane fractions from Micrococcus lysodeikticus.

2. Isolation of DNA-membrane complex in Bacillus subtilis.

3. Some unique properties of the deoxyribonucleic acid-bearing portion of the bacterial membrane.

4. Turnover of phosphatidylglycerol in Escherichia coli.

5. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.

6. Effect of different treatments on the activity of the heat labile enterotoxin of Escherichia coli F11(P155).

7. Metabolism of phosphatidylglycerol, phosphatidylethanolamine, and cardiolipin of Bacillus stearothermophilus.